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Keratin 5 (KRT5) antibody (Biotin)

Details for Product No. ABIN112168
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Antigen
Synonyms CK5, DDD, DDD1, EBS2, K5, KRT5A, Tfip8, Krt2-5, AW146334, 3300001P10Rik, Sak57, ckii, wu:fb02h09, wu:fj02d02, wu:fk78b12, zfCKII, KRT75
Reactivity
Hydra, Amphibian, Cow (Bovine), Human, Mouse (Murine), Pig (Porcine)
(166), (62), (47), (29), (28), (27), (4), (2), (2), (2), (2), (1)
Host
Mouse
(106), (69), (4)
Clonality (Clone)
Monoclonal ()
Conjugate
Biotin
(8), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(122), (81), (80), (50), (26), (21), (21), (20), (18), (4), (3), (1)
Pubmed 3 references available
Quantity 0.25 mL
Shipping to United States (Change)
Availability Will be delivered in 6 to 8 Business Days
Catalog No. ABIN112168
478.50 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Human keratin K8, purified from SDS PAGE gel
Clone C22
Isotype IgG1
Specificity Antibody clone C22 represents an excellent marker for distinguishing carcinomas from all non-epithelial tumors. The antibody specifically reacts with Keratins K5 and K8 present in nearly all epithelia. Polypeptide Reacting: Mr 52 500, Mr 58 000 keratins (type II keratins K5 and K8, formerly also designated cytokeratins 5 and 8) of human epithelial cells. Reactivities on Cultured Cell Lines (tested so far): MCF-7, RT 112, HT-29, Detroit 562, RPMI 2650, SSC-12, bovine BMGE+H, BMGE-H, MDBK. Epitope has been mapped to aa 353-367 on alpha helical rod domain (See Waseem et al., 2004).
Purification Affinity Chromatography on Protein A
Alternative Name Cytokeratin 5
Background Cytokeratin 5 is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. Cytokeratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.
Alternate names: 58 kDa Cytokeratin, CK5, Cytokeratin-5, K5, KRT5, Keratin 5, Keratin type II cytoskeletal 5, Keratin-5
Gene ID 3852
NCBI Accession NP_000415
UniProt P13647
Research Area Signaling, Cytoskeleton, Cytokeratins, Extracellular Matrix
Application Notes ELISA. Flow Cytometry. Immunocytology. Immunohistochemistry on Frozen Tissues (1/10-1/20), Incubation time: 1 h at RT. Immunohistochemistry on Paraffin-Embedded Tissues (1/10-1/20), Incubation time: overnight at 2-8°C. With paraffin-embedded sections, protease pretreatment is requiredprior to antibody application.
Restrictions For Research Use only
Format Liquid
Buffer 0.09% Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Background publications Bártek, Vojtĕsek, Stasková et al.: "A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology." in: The Journal of pathology, Vol. 164, Issue 3, pp. 215-24, 1991 (PubMed).

Waseem, Karsten, Leigh et al.: "Conformational changes in the rod domain of human keratin 8 following heterotypic association with keratin 18 and its implication for filament stability." in: Biochemistry, Vol. 43, Issue 5, pp. 1283-95, 2004 (PubMed).

General Moll, Franke, Schiller et al.: "The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells." in: Cell, Vol. 31, Issue 1, pp. 11-24, 1983 (PubMed).

Hosts (106), (69), (4)
Reactivities (166), (62), (47), (29), (28), (27), (4), (2), (2), (2), (2), (1)
Applications (122), (81), (80), (50), (26), (21), (21), (20), (18), (4), (3), (1)
Conjugates (8), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Epitopes (24), (7), (3), (2), (2), (2), (2), (2), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN112168
478.50 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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