Keratin 5 (KRT5) antibody (Biotin)

Antigen: Keratin 5 (KRT5)

Synonyms: K5, CK5, DDD, EBS2, KRT5A, Tfip8, Krt2-5, AW146334, 3300001P10Rik, Sak57, KRT79, KRT5, ckii, zfCKII, MGC77647, wu:fb02h09, wu:fj02d02, wu:fk78b12, KRT75

Clonality: Monoclonal (C22)

Host: Mouse

Reactivity: Hydra, Amphibian, Cow (Bovine), Human, Mouse (Murine), Pig (Porcine)

Conjugate: Biotin

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN112168

Additional Information

Alternative name: Cytokeratin 5

Gene ID: 3852

UniProt: P13647

Immunogen: Human keratin K8, purified from SDS PAGE gel

Cross-Reactivity: Amphibian, Cow (Bovine), Human, Mouse (Murine), Pig (Porcine)

Isotype: IgG1

Clone: C22

Description: Cytokeratin 5 is a member of the keratin gene family. The type II cytokeratins consist ofbasic or neutral proteins which are arranged in pairs of heterotypic keratin chainscoexpressed during differentiation of simple and stratified epithelial tissues. This type IIcytokeratin is specifically expressed in the basal layer of the epidermis with family memberKRT14. Mutations in these genes have been associated with a complex of diseases termedepidermolysis bullosa simplex. The type II cytokeratins are clustered in a region ofchromosome 12q12-q13. Cytokeratin 8 is a member of the type II keratin family clustered on the long arm ofchromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sizedfilaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizeswith keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions insignal transduction and cellular differentiation. Mutations in this gene cause cryptogeniccirrhosis.

Characteristics: Synonyms: KRT5, Cytokeratin-5, Keratin-5, Keratin 5, CK5, K5, Keratin type II cytoskeletal 5, 58 kDaCytokeratin

Application Details

Application Notes: ELISA. Flow Cytometry. Immunocytology. Immunohistochemistry on Frozen Tissues (1/10-1/20), Incubation time: 1 h at RT. Immunohistochemistry on Paraffin-Embedded Tissues (1/10-1/20), Incubation time: overnight at 2-8°C. With paraffin-embedded sections, protease pretreatment is requiredprior to antibody application.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Research Area: Signaling, Cytoskeleton, Cytokeratins, Extracellular Matrix

Restrictions: For Research Use only

Publications

Bártek, Vojtĕsek, Stasková et al.: "A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology." in: The Journal of pathology, Vol. 164, Issue 3, pp. 215-24, 1991 (PubMed).

Moll, Franke, Schiller et al.: "The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells." in: Cell, Vol. 31, Issue 1, pp. 11-24, 1983 (PubMed).