Desmocollin 3 (DSC3) antibody

Details for Product No. ABIN112239
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Antigen
Synonyms dsc3, MGC85083, CDHF3, DSC, DSC1, DSC2, DSC4, HT-CP, 5430426I24Rik
Reactivity
Human
(47), (21), (15), (14), (14)
Host
Mouse
(30), (17), (2)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(39), (16), (13), (10), (9), (9), (4), (4), (3), (1), (1)
Pubmed 8 references available
Quantity 50 μg
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Catalog No. ABIN112239
456.50 $
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Immunogen Synthetic peptide corresponding to a sequence present on the extracellular anchor domain of Human Desmocollin 3.
Sequence VHGAPFYFSL PNTSPEISRL WSLTKVC
Clone Dsc3-U114
Isotype IgG1
Specificity This anibody localizes Dsc3 present in: - all living epidermal layers - in glandular duct cells - in basal matrix cells and outer root sheath of hair follicles - basal and suprabasal cell layers of stratified epithelia (e.g. vagina, tongue, esophagus), basal layer of bladder urothelium and complex epithelium of trachea - thymic reticulum cells All simple epithelia are negative. Intercalated disks of myocardium are negative. Polypeptide reacting: 109 kDa and 100 kDa polypeptides (desmocollin 3 splice isoforms at pI 5.2) from human epidermal desmosomes. Most tested cultured cell lines derivated from stratified squamous epithelia or squamous cell carcinoma are positive (e.g A-431, HaCat). Does not react with Bovine and Rodent.
Purification Affinity Chromatography on Protein A
Alternative Name Desmocollin-3
Background Desmocollin 3 is a calcium-dependent glycoprotein that is a member of the desmocollin subfamily of the cadherin superfamily. These desmosomal family members, along with the desmogleins, are found primarily in epithelial cells where they constitute the adhesive proteins of the desmosome cell-cell junction and are required for cell adhesion and desmosome formation. The desmosomal family members are arranged in two clusters on chromosome 18, occupying less than 650 kb combined.Synonyms: CDHF3, Cadherin family member 3, DSC3, DSC4, Desmocollin-4, HT-CP
Gene ID 1825
NCBI Accession NP_001932
UniProt Q14574
Research Area Cytoskeleton
Application Notes Immunoblotting (Western). Immunohistochemistry on Frozen Sctions. Immunohistochemistry on Paraffin-Embedded Sections after microwave treatment: 1/10 inPBS ( pH 7.4) for 1h at RT. Recommendations: For better resolution on Frozen Sections preincubation (directly afterfixation) with 0.05-0.2 % Triton X100, for 5-10 min, depending on tissue type, isrecommended (Ref.2).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Reconstitution Restore in 1 mL distilled water.
Buffer PBS buffer, pH 7.4, 0.09 % Sodium Azide, 0.5 % BSA
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice This product is photosensitive and should be protected from light
Storage 4 °C
Storage Comment Prior to and following reconstitution store the antibody undiluted at 2-8 °C. DO NOT FREEZE!
Product cited in: Hamidov, Altendorf-Hofmann, Chen et al.: "Reduced expression of desmocollin 2 is an independent prognostic biomarker for shorter patients survival in pancreatic ductal adenocarcinoma." in: Journal of clinical pathology, Vol. 64, Issue 11, pp. 990-4, 2011 (PubMed).

Background publications Schäfer, Stumpp, Franke: "Immunological identification and characterization of the desmosomal cadherin Dsg2 in coupled and uncoupled epithelial cells and in human tissues." in: Differentiation; research in biological diversity, Vol. 60, Issue 2, pp. 99-108, 1996 (PubMed).

Nuber, Schäfer, Stehr et al.: "Patterns of desmocollin synthesis in human epithelia: immunolocalization of desmocollins 1 and 3 in special epithelia and in cultured cells." in: European journal of cell biology, Vol. 71, Issue 1, pp. 1-13, 1997 (PubMed).

Kurzen, Moll, Moll et al.: "Compositionally different desmosomes in the various compartments of the human hair follicle." in: Differentiation; research in biological diversity, Vol. 63, Issue 5, pp. 295-304, 1998 (PubMed).

Moll, Houdek, Schäfer et al.: "Diversity of desmosomal proteins in regenerating epidermis: immunohistochemical study using a human skin organ culture model." in: Archives of dermatological research, Vol. 291, Issue 7-8, pp. 437-46, 1999 (PubMed).

Hatzfeld, Haffner, Schulze et al.: "The function of plakophilin 1 in desmosome assembly and actin filament organization." in: The Journal of cell biology, Vol. 149, Issue 1, pp. 209-22, 2000 (PubMed).

South, Wan, Stone et al.: "Lack of plakophilin 1 increases keratinocyte migration and reduces desmosome stability." in: Journal of cell science, Vol. 116, Issue Pt 16, pp. 3303-14, 2003 (PubMed).

Akat, Mennel, Kremer et al.: "Molecular characterization of desmosomes in meningiomas and arachnoidal tissue." in: Acta neuropathologica, Vol. 106, Issue 4, pp. 337-47, 2003 (PubMed).

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