You are viewing an incomplete version of our website. Please click to reload the website as full version.

Keratin 3 (KRT3) antibody

Details for Product No. ABIN112318, Supplier: Login to see New
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Synonyms B-CK, Bck, Ck-3, Ck3, Ckbb, CK3, K3, CK-3
Cow (Bovine), Human, Rabbit
(22), (3), (1), (1), (1)
(19), (2), (1)
Clonality (Clone)
Monoclonal ()
(1), (1), (1), (1), (1), (1), (1)
Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
(17), (9), (2), (2), (2), (2), (1), (1), (1), (1)
Pubmed 3 references available
Supplier Login to see New
Catalog number from supplier Login to see New
Quantity 0.2 mg
Shipping to United States ( )
Availability Will be delivered in 6 to 8 Business Days
Immunogen Human epithelial keratin
Clone AE5
Isotype IgG1
Specificity Cytokeratin 3 antibody, clone AE5 represents an excellent marker for corneal type differentiation. Positive for epithelial cells of cornea, snout and some oral mucosa. This antibody has been used for studying corneal epithelial stem cells. Polypeptide Reacting: Mr 64 000 polypeptide (Keratin K3, formerly also designated Cytokeratin 3) of human corneal epithelium and Keratin K76 (formerly also designated Cytokeratin K2p) of palate epithelium. Reactivities on Cultured Cell Lines: Rabbit corneal epithelial cells. In Cow and Rabbit it reacts with lip and snout epithelia.
Cross-Reactivity (Details) Species reactivity (tested):Human, Rabbit and Bovine.
Purification Ion Exchange Chromatography.
Alternative Name Cytokeratin 3 (KRT3 Antibody Abstract)
Background Cytokeratin 3 belongs to the intermediate filament family. It is a type II cytokeratin and is specifically expressed in the corneal epithelium. Cytokeratin 3 associates with Cytokeratin 12. Defects in Cytokeratin 3 are a cause of Meesmann corneal dystrophy.Synonyms: 65 kDa cytokeratin, KRT3, Keratin, Type-II keratin Kb3, keratin-3, keratin-76, type II cytoskeletal 3
Gene ID 3850
NCBI Accession NP_476429
UniProt P12035
Application Notes Immunoblotting: 1/500. Immunohistochemistry on Frozen Sections: 1/50 (1h at RT).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS, pH 7,2 containing 0.09 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Background publications Moll, Franke, Schiller et al.: "The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells." in: Cell, Vol. 31, Issue 1, pp. 11-24, 1983 (PubMed).

Cooper, Schermer, Sun: "Classification of human epithelia and their neoplasms using monoclonal antibodies to keratins: strategies, applications, and limitations." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 52, Issue 3, pp. 243-56, 1985 (PubMed).

Schermer, Galvin, Sun: "Differentiation-related expression of a major 64K corneal keratin in vivo and in culture suggests limbal location of corneal epithelial stem cells." in: The Journal of cell biology, Vol. 103, Issue 1, pp. 49-62, 1986 (PubMed).

Catalog No. ABIN112318
Plus shipping costs $45.00

Order hotline:

  • +1 877 302 8632
  • +1 888 205 9894 (TF)