Keratin 3 (KRT3) antibody

Details for Product No. ABIN112318
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Antigen
Synonyms B-CK, Bck, Ck-3, Ck3, Ckbb, CK3, K3, CK-3
Reactivity
Cow (Bovine), Human, Rabbit
(21), (13), (13), (12), (12), (12), (1)
Host
Mouse
(17), (3), (2)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
(11), (10), (5), (2), (1), (1), (1), (1), (1)
Pubmed 3 references available
Quantity 0.2 mg
Shipping to United States (Change)
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Catalog No. ABIN112318
643.50 $
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Immunogen Human epithelial keratin
Clone AE5
Isotype IgG1
Specificity Cytokeratin 3 antibody, clone AE5 represents an excellent marker for corneal type differentiation. Positive for epithelial cells of cornea, snout and some oral mucosa. This antibody has been used for studying corneal epithelial stem cells. Polypeptide Reacting: Mr 64 000 polypeptide (Keratin K3, formerly also designated Cytokeratin 3) of human corneal epithelium and Keratin K76 (formerly also designated Cytokeratin K2p) of palate epithelium. Reactivities on Cultured Cell Lines: Rabbit corneal epithelial cells. In Cow and Rabbit it reacts with lip and snout epithelia.
Cross-Reactivity (Details) Species reactivity (tested):Human, Rabbit and Bovine.
Purification Ion Exchange Chromatography.
Alternative Name Cytokeratin 3
Background Cytokeratin 3 belongs to the intermediate filament family. It is a type II cytokeratin and is specifically expressed in the corneal epithelium. Cytokeratin 3 associates with Cytokeratin 12. Defects in Cytokeratin 3 are a cause of Meesmann corneal dystrophy.Synonyms: 65 kDa cytokeratin, KRT3, Keratin, Type-II keratin Kb3, keratin-3, keratin-76, type II cytoskeletal 3
Gene ID 3850
NCBI Accession NP_476429
UniProt P12035
Application Notes Immunoblotting: 1/500. Immunohistochemistry on Frozen Sections: 1/50 (1h at RT).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS, pH 7,2 containing 0.09 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Background publications Schermer, Galvin, Sun: "Differentiation-related expression of a major 64K corneal keratin in vivo and in culture suggests limbal location of corneal epithelial stem cells." in: The Journal of cell biology, Vol. 103, Issue 1, pp. 49-62, 1986 (PubMed).

Cooper, Schermer, Sun: "Classification of human epithelia and their neoplasms using monoclonal antibodies to keratins: strategies, applications, and limitations." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 52, Issue 3, pp. 243-56, 1985 (PubMed).

Moll, Franke, Schiller et al.: "The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells." in: Cell, Vol. 31, Issue 1, pp. 11-24, 1983 (PubMed).

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