Keratin 13 (KRT13) antibody

Antigen: Keratin 13 (KRT13)

Synonyms: K13, CK13, MGC3781, MGC161462, Krt1-13, Krt-1.13, Ka13, k13, ck13, MGC84388, MGC115054, krt13, KRT15

Clonality: Monoclonal (Ks13-1)

Host: Mouse

Reactivity: Cow (Bovine), Human, Rat (Rattus)

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN112324

Additional Information

Alternative name: Cytokeratin 13

UniProt: P13646

Immunogen: Keratin K13 of Mr 54. 000 purified from human esophagus.

Cross-Reactivity: Cow (Bovine), Human, Rat (Rattus)

Isotype: IgG1

Clone: Ks13-1

Description: Cytokeratin 13 is a member of the keratin gene family. The keratins are intermediatefilament proteins responsible for the structural integrity of epithelial cells and aresubdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist ofacidic proteins which are arranged in pairs of heterotypic keratin chains. This type Icytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornifiedstratified epithelia. Mutations in this gene and keratin 4 have been associated with theautosomal dominant disorder White Sponge Nevus.

Characteristics: Synonyms: CK-13, CK13, Keratin-13, KRT13, K13, KRT-13, Keratin type I cytoskeletal 13

Application Details

Application Notes: Immunofluorescence. Immunohistochemistry on frozen and paraffin sections: 1: 10, protease pretreatmentimproves the performance of this excellent marker in immunohisto-chemistry: 1/10 withPBS, pH 7.4. Western Blot. Incubation time: 1h at RT, extended with parafin. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Purification: Purified

Buffer: Contains 0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4Reconstitution: Restore in 1 ml dist. water.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Research Area: Signaling, Cytoskeleton, Extracellular Matrix

Restrictions: For Research Use only

Publications

Dallenbach-Hellweg, Lang: "Immunohistochemical studies on uterine tumors. I. Invasive squamous cell carcinomas of the cervix and their precursors." in: Pathology, research and practice, Vol. 187, Issue 1, pp. 36-43, 1991 (PubMed).

Moll, Achtstaetter, Becht et al.: "Cytokeratins in normal and malignant transitional epithelium. Maintenance of expression of urothelial differentiation features in transitional cell carcinomas and bladder carcinoma cell culture lines." in: The American journal of pathology, Vol. 132, Issue 1, pp. 123-44, 1988 (PubMed).

Moll, Franke, Schiller et al.: "The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells." in: Cell, Vol. 31, Issue 1, pp. 11-24, 1983 (PubMed).

Demirkesen, Hoede, Moll: "Epithelial markers and differentiation in adnexal neoplasms of the skin: an immunohistochemical study including individual cytokeratins." in: Journal of cutaneous pathology, Vol. 22, Issue 6, pp. 518-35, 1996 (PubMed).