Selectin E/CD62e antibody

Catalog No. ABIN112381

Product Details anti-Selectin E/CD62e Antibody

Target: Selectin E/CD62e (SELE) (Selectin E (SELE))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Selectin E/CD62e antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Specificity: UZ6 recognizes a conformational epitope in the CR2 region in murine E-selectin. UZ6 partially inhibits leukocyte adhesion to murine activated endothelial cells.

Purification: Purified

Immunogen: LPS-activated mlEND1 cells expressing E-selectin at their cell surface.

Clone: UZ6

Isotype: IgG2b

Application Details

Application Notes: This antibody is suitable for Immunoblotting, ELISA, Immunoprecipitation,Immunohistochemistry on fozen sections and Flow cytometry (1/500).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS with 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store lyophilized at 2-8 °C. After reconstitution aliquot and store at -20 °C.

Target Details for Selectin E/CD62e

Target: Selectin E/CD62e (SELE) (Selectin E (SELE))

Alternative Name: CD62E / E-Selectin (SELE Products)

Background: Leukocytes adhere to the blood vessel endothelium during extravasation in postcapillary venules of lymph nodes. In addition, leukocyte adhesion occurs in the capillaries and small venules at any site in the body after onset of inflammation. This response is immediate and involves a cascade of adhesion receptors. At the endothelial surface members of the selectin and immunoglobulin superfamilies participate in this cascade. Selectins are C-type cell surface lectins that play a role in leukocyte adhesion to the blood vessel wall endothelium. E-selectin (CD62E) is an endothelial cell specific selectin that is expressed only after activation with proinflammatory cytokines. In vitro experiments have shown that IL-1, TNFalpha and bacterial wall components like lipopolysaccharides induce the transcription of E-selectin in a NFkB dependent signalling cascade. E-selectin has been associated with blood vessel endothelium in diverse inflammatory situations.Synonyms: CD62 antigen-like family member E, ELAM1, Endothelial leukocyte adhesion molecule 1, LECAM2, Leukocyte-endothelial cell adhesion molecule 2, SELE

Gene ID: 20339

NCBI Accession: NP_035475

UniProt: Q00690

Pathways: Thromboxane A2 Receptor Signaling