Epithelial Cell Adhesion Molecule (EPCAM) antibody

Antigen: Epithelial Cell Adhesion Molecule (EPCAM)

Synonyms: EGP, ESA, KSA, M4S1, MK-1, EGP-2, EGP34, EGP40, KS1/4, MIC18, TROP1, CO-17A, Ep-CAM, hEGP-2, CO17-1A, GA733-2, TACST-1, TACSTD1, sb:cb6, tacstd, zgc:77119, wu:fj17g02, EPCAM, tacstd1, MGC80540, MGC127627, egp, ksa, m4s1, mk-1, cd326, egp40, mic18, trop1, ep-cam, hegp-2, co17-1a, ga733-2

Clonality: Monoclonal (MK-1-25)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Catalog no.: ABIN114701

Additional Information

Alternative name: CD326 / EPCAM / TACSTD1

UniProt: P16422

Immunogen: The Human Ep-CAM is a recombinant protein produced in E. coli.

Cross-Reactivity: Human

Isotype: IgG1

Clone: MK-1-25

Description: The Ep-CAM antigen (Epithelial Cell Adhesion Molecule), also termed as MK-1, GA733-2,KSA, 17-1A or EGP40, is a 40 kDa type-I transmembrane glycoprotein with properties ofCa2+ -independent homotypic intracellular adhesion molecule. The full leghth of themolecule is 314 amino acids. Structurally, Ep-CAM is not related to any of the majorfamilies of adhesion molecules. The Ep-CAM antigen is overexpessed on the majority oftumor cells of most human epithelia of variety of tumor tissues such as stomach, colon,pancreas, gall bladder, bile duct, mammary gland, breast and lung carcinoma. Ep-CAM hasbeen suggested to be involved in the differentiation and growth of epithelial cells undernormal physiological conditions through its homotypic cell-cell adhesion activity. As it isoverexpressed on most carcinomas, Ep-CAM antigen has been used as a target fordiagnosis and therapy of cancer.

Characteristics: Synonyms: Ep-CAM, Epithelial cell adhesion molecule, GA733-2, EGP314, KSA, TROP1, Trop-1, M1S2,M4S1, MIC18, Tumor-associated calcium signal transducer 1, Major gastrointestinaltumor-associated protein GA733-2, Epithelial glycoprotein 314,Adenocarcinoma-associated antigen, KS 1/4 antigen

Specificity: The Anti Human Ep-CAM Antibody, Clone: MK-1-25 is a mouse monoclonal antibody againstrecombinant Human Ep-CAM. Species: Human. Others not tested.

Synonyms: EGP, ESA, KSA, M4S1, MK-1, EGP-2, EGP34, EGP40, KS1/4, MIC18, TROP1, CO-17A, Ep-CAM, hEGP-2, CO17-1A, GA733-2, TACST-1, TACSTD1, sb:cb6, tacstd, zgc:77119, wu:fj17g02, EPCAM, tacstd1, MGC80540, MGC127627, egp, ksa, m4s1, mk-1, cd326, egp40, mic18, trop1, ep-cam, hegp-2, co17-1a, ga733-2

Application Details

Application Notes: ELISA. Western Blot. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Purification: Purified

Buffer: 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2. AZIDE FREEReconstitution: Restore with 0.1 ml of deionized water

Storage: Store lyophilized (preferably in a desiccator) at -20°C and in aliquots at -80°C. Reconstituted antibody can be stored at 4°C for a limited period of time, it does not showdecline in activity after two weeks at 4°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Research Area: Cancer

Restrictions: For Research Use only

Publications

Balzar, Winter, de Boer et al.: "The biology of the 17-1A antigen (Ep-CAM)." in: Journal of molecular medicine (Berlin, Germany), Vol. 77, Issue 10, pp. 699-712, 2000 (PubMed).

Balzar, Briaire-de Bruijn, Rees-Bakker et al.: "Epidermal growth factor-like repeats mediate lateral and reciprocal interactions of Ep-CAM molecules in homophilic adhesions." in: Molecular and cellular biology, Vol. 21, Issue 7, pp. 2570-80, 2001 (PubMed).

Abe, Kuroki, Imakiire et al.: "Preparation of recombinant MK-1/Ep-CAM and establishment of an ELISA system for determining soluble MK-1/Ep-CAM levels in sera of cancer patients." in: Journal of immunological methods, Vol. 270, Issue 2, pp. 227-33, 2002 (PubMed).