Podoplanin (PDPN) antibody

Antigen: Podoplanin (PDPN)

Synonyms: T1A, GP36, GP40, Gp38, OTS8, T1A-2, AGGRUS, HT1A-1, PA2.26, T1a, OTS-8, T1alpha, RANDAM-2, E11, RTI40, T1-alpha, PDPN

Clonality: Monoclonal (18H5)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN115146

Additional Information

Alternative name: Podoplanin

UniProt: Q86YL7

Immunogen: GP36 (podoplanin) expressed by MDCK cells.

Cross-Reactivity: Human

Isotype: IgG1

Clone: 18H5

Description: Podoplanin (PDPN) is a small mucin-like transmembrane protein, widely expressed invarious specialized cell types throughout the body. This is a type-I integral membraneglycoprotein with diverse distribution in human tissues. The physiological function of thisprotein may be related to its mucin-type character.

Characteristics: Synonyms: Glycoprotein 36, PA2.26 antigen, T1-alpha, Aggrus, PDPN, GP36, PSEC0003, PSEC0025

Application Details

Protocol: Sample protocol for Formalin-Fixed Paraffin-Embedded Sections (as a guide only)1) Put Paraffin sections (2-4 µm) onto a Poly-Lysin object holder2) Deparaffination: - Xylol (10 min)- Acetone (10 min)- Acetone / buffer mixture (10 min)- Rinse 3x with wash buffer3) Microwave: boil up 3x 10 min in the appropriate buffer (put the whole glass curette intothe microwave)a) EDTA buffer: 0,1 M EDTA in aqua dest, pH 8,0b) Citrate buffer/10 mM, pH 6,0A: citric acid, 19,2 g in 1L aqua destB: Na-Citrate, 29,41 g in 1L aqua dest9 ml stock solution A + 50 ml stock solution B, in 500 ml aqua dest (pH adjustment only insolution B)Cool down the sections to RT for 25 min in wash buffer4) Addition of primary ABs (dilute e. g. in ready to use buffer from DAKO [S3022])- Shortly drip off the sections on paper (take 3-4 sections at once)- Dust off the liquid beside the sections- Add primary Abs- incubate 0. 5 h at RT- Rinse 3x with wash buffer- Shortly drip off the sections5) Addition of the by-pass AB (monoclonal mouse-anti-rabbit IgG, e. g. DAKO [M0737] 1: 250)- incubate 0. 5 h at RT- Rinse 3x with wash buffer- Shortly drip off the sections6) Addition of anti-mouse-IgG from rabbit 1: - Incubate 0. 5 h at RT- Rinse 3x with wash buffer- Shortly drip off the sections7)Addition of the APAAP complexes- Incubate 0. 5 h at RT

Application Notes: FACS analysis (3-20 µg/ml). Immuofluorescence (1-2 µg/ml). Western blot analysis (1-2 µg/ml). Immunohistochemistry on Frozen and Paraffin Sections (6-30 µg/ml). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Purification: Affinity Purified

Buffer: PBS, pH 7.4 without preservatives or stabilizers.Reconstitution: Restore in sterile water to a concentration of > 0.5 mg/ml.

Storage: The lyophilized IgG is stable at 2-8°C for one month and for one year when kept at -20°C. When reconstituted in sterile water to a concentration of > 0. 5 mg/ml the antibody is stablefor six weeks at 2-8°C in the presence of a preservative. Reconstituted antibody can alsobe aliquotted and stored at -20°C to -70°C for 6 months without detectable loss of activity. Avoid repeated freezing and thawing.

Restrictions: For Research Use only

Images

FACS analysis of Podoplanin in human microvascular endothelial cells using antibody ABIN115146.

Publications

Breiteneder-Geleff, Matsui, Soleiman et al.: "Podoplanin, novel 43-kd membrane protein of glomerular epithelial cells, is down-regulated in puromycin nephrosis." in: The American journal of pathology, Vol. 151, Issue 4, pp. 1141-52, 1997 (PubMed).

Zimmer, Oeffner, Von Messling et al.: "Cloning and characterization of gp36, a human mucin-type glycoprotein preferentially expressed in vascular endothelium." in: The Biochemical journal, Vol. 341 ( Pt 2), pp. 277-84, 1999 (PubMed).

Schacht, Ramirez, Hong et al.: "T1alpha/podoplanin deficiency disrupts normal lymphatic vasculature formation and causes lymphedema." in: The EMBO journal, Vol. 22, Issue 14, pp. 3546-56, 2003 (PubMed).