Podoplanin antibody

Catalog No. ABIN115146

Product Details anti-Podoplanin Antibody

Target: Podoplanin (PDPN)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Podoplanin antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antibody reacts with Podoplanin (gp36).

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography on Protein G.

Immunogen: GP36 (Podoplanin) expressed by MDCK cells.

Clone: 18H5

Isotype: IgG1

Application Details

Application Notes: FACS analysis (3-20 μg/mL). Immuofluorescence (1-2 μg/mL). Western blot analysis (1-2 μg/mL). Immunohistochemistry on Frozen and Paraffin Sections (6-30 μg/mL).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: Sample protocol for Formalin-Fixed Paraffin-Embedded Sections (as a guide only)1) Put Paraffin sections (2-4 µm) onto a Poly-Lysin object holder2) Deparaffination: - Xylol (10 min)- Acetone (10 min)- Acetone / buffer mixture (10 min)- Rinse 3x with wash buffer3) Microwave: boil up 3x 10 min in the appropriate buffer (put the whole glass curette intothe microwave)a) EDTA buffer: 0,1 M EDTA in aqua dest, pH 8,0b) Citrate buffer/10 mM, pH 6,0A: citric acid, 19,2 g in 1L aqua destB: Na-Citrate, 29,41 g in 1L aqua dest9 ml stock solution A + 50 ml stock solution B, in 500 ml aqua dest (pH adjustment only insolution B)Cool down the sections to RT for 25 min in wash buffer4) Addition of primary ABs (dilute e. g. in ready to use buffer from DAKO [S3022])- Shortly drip off the sections on paper (take 3-4 sections at once)- Dust off the liquid beside the sections- Add primary Abs- incubate 0. 5 h at RT- Rinse 3x with wash buffer- Shortly drip off the sections5) Addition of the by-pass AB (monoclonal mouse-anti-rabbit IgG, e. g. DAKO [M0737] 1: 250)- incubate 0. 5 h at RT- Rinse 3x with wash buffer- Shortly drip off the sections6) Addition of anti-mouse-IgG from rabbit 1: - Incubate 0. 5 h at RT- Rinse 3x with wash buffer- Shortly drip off the sections7)Addition of the APAAP complexes- Incubate 0. 5 h at RT

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.4 without preservatives or stabilizers

Preservative: Without preservative

Handling Advice: This product is photosensitive and should be protected from light

Storage: 4 °C

Storage Comment: Prior to and following reconstitution store the antibody undiluted at 2-8 °C. DO NOT FREEZE!

References for anti-Podoplanin antibody (ABIN115146)

Birke, Lütjen-Drecoll, Kerjaschki, Birke: "Expression of podoplanin and other lymphatic markers in the human anterior eye segment." in: Investigative ophthalmology & visual science, Vol. 51, Issue 1, pp. 344-54, (2010) (PubMed).

Conrad, Niess, Huss, Huber, von Luettichau, Nelson, Ott, Jauch, Bruns: "Multipotent mesenchymal stem cells acquire a lymphendothelial phenotype and enhance lymphatic regeneration in vivo." in: Circulation, Vol. 119, Issue 2, pp. 281-9, (2009) (PubMed).

Turner, Langley, Johnston, Gentil, Ford, Wu, Graham, Sharpley, Slatko, Pearlman, Taylor: "Wolbachia lipoprotein stimulates innate and adaptive immunity through Toll-like receptors 2 and 6 to induce disease manifestations of filariasis." in: The Journal of biological chemistry, Vol. 284, Issue 33, pp. 22364-78, (2009) (PubMed).

Kilic, Oliveira-Ferrer, Neshat-Vahid, Irmak, Obst-Pernberg, Wurmbach, Loges, Kilic, Weil, Lauke, Tilki, Singer, Ergün: "Lymphatic reprogramming of microvascular endothelial cells by CEA-related cell adhesion molecule-1 via interaction with VEGFR-3 and Prox1." in: Blood, Vol. 110, Issue 13, pp. 4223-33, (2007) (PubMed).

Riesenberg, Weiler, Spring, Eder, Buchner, Popp, Castro, Kammerer, Takikawa, Hatz, Stief, Hofstetter, Zimmermann: "Expression of indoleamine 2,3-dioxygenase in tumor endothelial cells correlates with long-term survival of patients with renal cell carcinoma." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 13, Issue 23, pp. 6993-7002, (2007) (PubMed).

Target Details for Podoplanin

Target: Podoplanin (PDPN)

Alternative Name: Podoplanin (PDPN Products)

Synonyms: PDPN antibody, AGGRUS antibody, GP36 antibody, GP40 antibody, Gp38 antibody, HT1A-1 antibody, OTS8 antibody, PA2.26 antibody, T1A antibody, T1A-2 antibody, OTS-8 antibody, RANDAM-2 antibody, T1-alpha antibody, T1a antibody, T1alpha antibody, E11 antibody, RTI40 antibody, podoplanin antibody, PDPN antibody, Pdpn antibody

Background: Podoplanin, also known as glycoprotein 36 (gp36), PA2.26 antigen, T1alpha (T1A), and aggrus, is a 36 kDa type I transmembrane sialoglycoprotein and member of the Podoplanin family. Podoplanin has three potential splice variants, the longest of which is represented by a 238 amino acid (aa) precursor (NP_006465). It contains an undefined signal sequence, a 22 aa transmembrane segment (aa 207-228) and a short cytoplasmic tail (aa 229-238). The ECD contains abundant Ser/Thr residues that could serve as potential O-linked glycosolation sites. The cytoplasmic tail contains putative sites for protein kinase C phosphorylation. There are two potential alternate start sites at Met 77 (Swiss Prot #: Q86YL7) and Met 119 (EAW51692) that generate short forms. The 162 aa short form Podoplanin precursor shares 47 % aa identity with mouse Podoplanin. Podoplanin is expressed on glomerular epithelial cells (podocytes), type I lung alveolar cells, lymphatic endothelial cells, and numerous tumors, including colorectal tumors, squamous cell carcinomas, testicular seminoma, and brain tumors. One study shows high expression of Podoplanin mRNA in placenta, lung, skeletal muscle, and heart, and weaker levels in brain, kidney, and liver. Podoplanin is the ligand for C-type lectin-like receptor 2 (CLEC2). Their association is dependent on sialic acid on O-glycans of Podoplanin. Through its association with CLEC2, Podoplanin-induces platelet aggregation and tumor metastasis. Podoplanin is also necessary for lymphatic vessel formation, normal lung cell proliferation and alveolus formation at birth.Synonyms: Aggrus, GP36, Glycoprotein 36, PA2.26 antigen, PDPN, PSEC0003, PSEC0025, T1-alpha

Gene ID: 10630

NCBI Accession: NP_001006625

UniProt: Q86YL7

Pathways: Dicarboxylic Acid Transport