Recombinant Podoplanin antibody (AA 1-100)

Catalog No. ABIN7504147

The Rabbit Monoclonal anti-Podoplanin antibody (Clone PDPN-4009R) (ABIN7504147) specifically detects Podoplanin in WB, ELISA and IF. The antibody is reactive with Human samples.

Quick Overview for Recombinant Podoplanin antibody (AA 1-100) (ABIN7504147)

Target: Podoplanin (PDPN)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Podoplanin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Clone: PDPN-4009R

Product Details anti-Podoplanin Antibody

Binding Specificity: AA 1-100

Purpose: Recombinant Podoplanin (PDPN) (Lymphatic Endothelial & Mesothelial Marker) Antibody

Specificity: It recognizes a muco-protein of 38-43 kDa, which is identified as Podoplanin (PDPN). It localizes in stromal cells of peripheral lymphoid tissue and thymic epithelial cells. As a regulator of the lymphatic endothelium, podoplanin probably plays a role in maintaining the unique shape of podocytes. It is selectively expressed in lymphatic endotheliumas well as lymphoangiomas,Kaposi sarcomas,and in a subset of angiosarcomas with probable lymphatic differentiation. Recent studies have also shown podoplanin to be a highly sensitive and relatively specific marker for epithelioid mesothelioma. Therefore, it can be used in a panel to distinguish mesotheliomas or mesothelial cells from pulmonary carcinomas.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Synthetic peptide corresponding to residues within aa1-100 of Podoplanin

Isotype: IgG, kappa

Application Details

Application Notes:

Positive Control: Human tonsil, cervical or lung carcinoma tissues.

Known Application: ELISA (For coating, order antibody without BSA), Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Podoplanin

Target: Podoplanin (PDPN)

Alternative Name: PDPN

Background:

Aggrus, Glycoprotein 36 KD, Glycoprotein 36, gp36, GP38, GP40, HT1A1, hT1alpha1, hT1alpha2, Lung type I cell membrane associated glycoprotein, Lung type I cell membrane associated glycoprotein T1A 2, OTS8,

cervical or lung carcinoma tissues., Human tonsil

Molecular Weight: 17kDa

Gene ID: 10630, 468675

UniProt: Q86YL7

Pathways: Dicarboxylic Acid Transport