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Antigen Identified By Monoclonal Antibody Ki-67 (MKI67) antibody

Details for Product No. ABIN115328
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Antigen
Synonyms MKI67, kia, mki67, MGC132156, D630048A14Rik, Ki-67, Ki67, KIA, MIB-1
Reactivity
Human
(192), (30), (27), (2), (1), (1), (1)
Host
Rabbit
(118), (75), (6), (2), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(91), (68), (59), (54), (33), (29), (18), (10), (9), (1), (1), (1)
Pubmed 2 references available
Quantity 1 mL
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Catalog No. ABIN115328
467.50 $
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Immunogen Synthetic peptide from 62 base pair region of the human Ki-67 antigen.
Specificity This antibody reacts with a nuclear antigen present in proliferating human cells. It can be used to evaluate the ki-67 labeling index in various tumors. Cellular Localization: Nuclear.
Purification Purified
Alternative Name Ki-67
Background Ki-67 antigen is expressed in proliferating cells but not in quiescent cells. Expression of this antigen occurs preferentially during late G1, S, G2, and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected. Consequently, Ki-67 antigen expression is used in tumor pathology to detect proliferating cells in neoplastic diseases. In cultured cells, Ki-67 is expressed in the nucleolus of interphase cells. The Ki-67 gene contains 15 exons. The Ki-67 repeat region, within which there is a 22-amino acid Ki-67 motif, is encoded by exon 13. The shorter isoform lacks exon 7. Northern blot analysis re- vealed multiple transcripts ranging from approximately 8.9 to 12.5 kb in proliferating but not quies-cent cells. Immunoblot analysis showed expression of 320- and 359-kD proteins. Sequence analy-sis predicted that the short-lived 2,896- and 3,256-amino acid protein isoforms contain potential nuclear targeting signals, over 200 potential phosphorylation sites, 19 N-myristoylation sites, 3 amidation sites, and numerous PEST sites. Antisense oligonucleotides inhibited cellular prolifera-tion in a dose-dependent manner, suggesting that Ki-67 protein expression may be an absolute requirement for cell proliferation.
Alternate names: Ki67 antigen, MKI67, Proliferation marker
Gene ID 4288
NCBI Accession NP_001139438.1
UniProt P46013
Research Area Proliferation Markers, Cell Cycle
Application Notes Immunohistochemsistry on Frozen and Formalin-Fixed Paraffin Embedded Sections: Use adilution of 1/25-1/50 in an ABC method (30 minutes at room temperature). Formalin fixed paraffin embedded tissue sections require high temperature antigenunmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Recommended Positive Control: Tonsil. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Supplier Images
anti-Antigen Identified By Monoclonal Antibody Ki-67 (MKI67) antibody Formalin fixed paraffin embedded human tonsil stained with Ki-67 antibody. ABIN115328
General Stepanova, Leng, Parker et al.: "Mammalian p50Cdc37 is a protein kinase-targeting subunit of Hsp90 that binds and stabilizes Cdk4." in: Genes & development, Vol. 10, Issue 12, pp. 1491-502, 1996 (PubMed).

Huang, Grammatikakis, Toole: "Organization of the chick CDC37 gene." in: The Journal of biological chemistry, Vol. 273, Issue 6, pp. 3598-603, 1998 (PubMed).

Validation Images
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