Antigen Identified By Monoclonal antibody Ki-67 (MKI67) antibody

Antigen: Antigen Identified By Monoclonal Antibody Ki-67 (MKI67)

Synonyms: KIA, Ki67, Ki-67, D630048A14Rik, MKI67, kia, mki67, MGC132156

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)

Catalog no.: ABIN115328

Additional Information

Alternative name: Ki-67

UniProt: P46013

Immunogen: Synthetic peptide from 62 base pair region of the human Ki-67 antigen.

Cross-Reactivity: Human

Description: Ki-67 antigen is expressed in proliferating cells but not in quiescent cells. Expression ofthis antigen occurs preferentially during late G1, S, G2, and M phases of the cell cycle,while in cells in G0 phase the antigen cannot be detected. Consequently, Ki-67 antigenexpression is used in tumor pathology to detect proliferating cells in neoplastic diseases. In cultured cells, Ki-67 is expressed in the nucleolus of interphase cells. The Ki-67 genecontains 15 exons. The Ki-67 repeat region, within which there is a 22-amino acid Ki-67motif, is encoded by exon 13. The shorter isoform lacks exon 7. Northern blot analysisre-vealed multiple transcripts ranging from approximately 8. 9 to 12. 5 kb in proliferating butnot quies-cent cells. Immunoblot analysis showed expression of 320- and 359-kD proteins. Sequence analy-sis predicted that the short-lived 2,896- and 3,256-amino acid proteinisoforms contain potential nuclear targeting signals, over 200 potential phosphorylationsites, 19 N-myristoylation sites, 3 amidation sites, and numerous PEST sites. Antisenseoligonucleotides inhibited cellular prolifera-tion in a dose-dependent manner, suggestingthat Ki-67 protein expression may be an absolute requirement for cell proliferation.

Characteristics: Synonyms: Ki67 antigen, MKI67, Proliferation marker

Specificity: This antibody reacts with a nuclear antigen present in proliferating human cells. It can be used to evaluate the ki-67 labeling index in various tumors. Cellular Localization: Nuclear. Species: Human. Others not tested.

Application Details

Application Notes: Immunohistochemsistry on Frozen and Formalin-Fixed Paraffin Embedded Sections: Use adilution of 1/25-1/50 in an ABC method (30 minutes at room temperature). Formalin fixed paraffin embedded tissue sections require high temperature antigenunmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Recommended Positive Control: Tonsil. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Purification: Purified

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Research Area: Proliferation Markers, Cell Cycle

Restrictions: For Research Use only

Images

Formalin fixed paraffin embedded human tonsil stained with Ki-67 antibody. ABIN115328

Publications

Stepanova, Leng, Parker et al.: "Mammalian p50Cdc37 is a protein kinase-targeting subunit of Hsp90 that binds and stabilizes Cdk4." in: Genes & development, Vol. 10, Issue 12, pp. 1491-502, 1996 (PubMed).

Huang, Grammatikakis, Toole: "Organization of the chick CDC37 gene." in: The Journal of biological chemistry, Vol. 273, Issue 6, pp. 3598-603, 1998 (PubMed).