Resistin (RETN) antibody

Details for Product No. ABIN115515
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Antigen
Reactivity
Human
(127), (55), (26), (12), (4), (1)
Host
Goat
(121), (33), (25), (5), (4), (4)
Clonality
Polyclonal
Conjugate
Un-conjugated
(21), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2)
Application
ELISA, Western Blotting (WB)
(129), (124), (23), (20), (13), (11), (9), (6), (4), (4), (4), (3), (2), (2), (1), (1), (1)
Pubmed 10 references available
Quantity 0.1 mg
Shipping to United States (Change)
Availability Discontinued
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Catalog No. ABIN115515
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Immunogen Recombinant Human Resistin (Source of antigen: E. coli)
Specificity The antibody recognizes human Resistin. Other species not tested.
Purification Immunoaffinity chromatography on a column with immobilized recombinant Human Resistin
Alternative Name Resistin
Background Resistin, a product of the RSTN gene, is a peptide hormone belonging to the class of cysteine-rich secreted proteins which is termed the RELM family, and is also described as ADSF (Adipose Tissue-Specific Secretory Factor) and FIZZ3 (Found in Inflammatory Zone). Human resistin contains 108 amino acids as a prepeptide, and its hydrofobic signal peptide is cleaved before its secretion. Resistin circulates in human blood as a dimeric protein consisting of two 92 amino acid polypeptides, which are disulfide-linked via Cys26. Resistin may be an important link between obesity and insulin resistance. Mouse resistin, specifically produced and secreted by adipocyte, acts on skeletal muscle myocytes, hepatocytes and adipocytes themselves so that it reduces their sensitivity to insulin. Steppan et al. have suggested that resistin suppresses the ability of insulin to stimulace glucose uptake. They have also suggested that resistin is present at elevated levels in blood of obese mice, and is down regulated by fasting and antidiabetic drugs. Way et al., on the other hand, have found that resistin expression is severly suppressed in obesity and is stimulated by several antidiabetic drugs. Other studies have shown that mouse resistin increases during the differentiation of adipocytes, but it also seems to inhibit adipogenesis. In contrast, the human adipogenic differentiation is likely to be associated with a down regulation of resistin gene expression. Recent studies have shown that human resistin is expressed also in macrophages and may be a novel link between inflammation and insulin resistance.
Alternate names: ADSF, Adipose tissue-specific secretory factor, C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein, Cysteine-rich secreted protein A12-alpha-like 2, Cysteine- rich secreted protein FIZZ3, FIZZ3, HXCP1, RETN, RSTN
Gene ID 56729
NCBI Accession NP_001180303
UniProt Q9HD89
Research Area Cardiovascular, Atherosclerosis, Metabolism, Hormones
Application Notes ELISA. Western blotting. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 0.1 mL of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.
Buffer Lyophilized from 1 mg/ml in 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2, AZIDE FREE
Preservative Sodium azide
Precaution of Use Do Not Use Sodium Azide as Preservative.
Handling Advice Avoid repeated freezing and thawing. Dilute only prior to immediate use
Storage 4 °C/-20 °C
Storage Comment Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8°C as an undiluted liquid.
Expiry Date 12 months
Background publications Steppan, Bailey, Bhat et al.: "The hormone resistin links obesity to diabetes." in: Nature, Vol. 409, Issue 6818, pp. 307-12, 2001 (PubMed).

Steppan, Brown, Wright et al.: "A family of tissue-specific resistin-like molecules." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 2, pp. 502-6, 2001 (PubMed).

Kim, Lee, Moon et al.: "A cysteine-rich adipose tissue-specific secretory factor inhibits adipocyte differentiation." in: The Journal of biological chemistry, Vol. 276, Issue 14, pp. 11252-6, 2001 (PubMed).

Banerjee, Lazar: "Dimerization of resistin and resistin-like molecules is determined by a single cysteine." in: The Journal of biological chemistry, Vol. 276, Issue 28, pp. 25970-3, 2001 (PubMed).

Way, Görgün, Tong et al.: "Adipose tissue resistin expression is severely suppressed in obesity and stimulated by peroxisome proliferator-activated receptor gamma agonists." in: The Journal of biological chemistry, Vol. 276, Issue 28, pp. 25651-3, 2001 (PubMed).

Juan, Au, Fang et al.: "Suppressed gene expression of adipocyte resistin in an insulin-resistant rat model probably by elevated free fatty acids." in: Biochemical and biophysical research communications, Vol. 289, Issue 5, pp. 1328-33, 2001 (PubMed).

Steppan, Lazar: "Resistin and obesity-associated insulin resistance." in: Trends in endocrinology and metabolism: TEM, Vol. 13, Issue 1, pp. 18-23, 2001 (PubMed).

Hartman, Hu, Tyler et al.: "Mechanisms regulating adipocyte expression of resistin." in: The Journal of biological chemistry, Vol. 277, Issue 22, pp. 19754-61, 2002 (PubMed).

Maebuchi, Machidori, Urade et al.: "Low resistin levels in adipose tissues and serum in high-fat fed mice and genetically obese mice: development of an ELISA system for quantification of resistin." in: Archives of biochemistry and biophysics, Vol. 416, Issue 2, pp. 164-70, 2003 (PubMed).

Pravenec, Kazdová, Landa et al.: "Transgenic and recombinant resistin impair skeletal muscle glucose metabolism in the spontaneously hypertensive rat." in: The Journal of biological chemistry, Vol. 278, Issue 46, pp. 45209-15, 2003 (PubMed).

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