Interleukin 1 alpha (IL1A) antibody

Antigen: Interleukin 1 alpha (IL1A)

Synonyms: IL1, IL-1A, IL1F1, IL1-ALPHA, Il-1a, IL-1 alpha, Il1a

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Catalog no.: ABIN116863

Additional Information

Alternative name: Interleukin-1 alpha / IL-1A

UniProt: P01583

Immunogen: The whole rabbit serum used to produce this IgG fraction antibody was prepared byrepeated immunizations with recombinant human IL-1a produced in E. coli. Remarks: The MW of the recombinant IL-1a was 17,000. This is the cleavage site generatedby the IL-1b converting enzyme (ICE, capase-1).

Cross-Reactivity: Human, Monkey

Description: Interleukins (ILs) are a large group of cytokines that are produced mainly by leukocytes,although some are produced by certain phagocytes and auxiliary cells. Each IL acts on aspecific, limited group of cells through a receptor specific for that IL. Interleukin 1 (IL1),originally known as lymphocyte activating factor (LAF), activates T cells and lymphocytes,which then proliferate and secrete interleukin 2. IL1 is primarily released from stimulatedmacrophages and monocytes, but also is released from several other cell types and isthought to play a key role in inflammatory and immune responses. The two closely relatedagents, interleukin 1 alpha (IL1 alpha) and interleukin 1 beta (IL1 beta) bind to the same cellsurface receptor, elicit nearly identical biological responses and share 25% homology intheir amino acid sequence.

Characteristics: Synonyms: IL-1 alpha, IL1F1, IL1A, Hematopoietin-1

Specificity: This antibody is primarily directed against the 17,000 MW human IL-1a and is useful indetermining its presence in various assays. In general, this antibody also detects primateIL-1a in the same formats using similar dilutions. The antiserum does not recognize humanIL-1b or Mouse or Rabbit IL-1a. In ELISA formats and other immunoreactive assays, thisantibody will recognize both the mature 17,000 MW IL-1a as well as the 31,000 MW IL-1aprecursor in either non-denatured (native) or denatured samples. Unlike the IL-1bprecursor, the native precursor of IL-1a is recognized by the antibody produced to the17,000 MW form. The 31,000 precursor of IL-1a is biologically active and is found primarilyintracellularly. The precursor of IL-1a, unlike that of IL-1b, is biologically active whenapplied to cells and is thought to have a role as a functional molecule intracellularly andcan be found constitutively expressed in various cell. This antibody is also useful forNeutralization of human and primate IL-1a activity in bioassays. It does not neutralize thebiological activity IL-1b. It does not neutralize the biological activity of mouse, rat or rabbitIL-1a. It will neutralize primate IL-a. For Neutralization, it is recommended to incubate thesample with a 1: 100 dilution of the antibody for at least 4 hours before being tested. Acontrol of similarly diluted normal rabbit IgG is recommended. This antibody can be usedfor FACS analysis. Caution should be exhibited as the F( c) domain of the rabbit IgGmolecule may interact with cells non-specifically. Add. Information: Endotoxin Content: < 10 pg/µl by LAL method.

Application Details

Application Notes: This IgG fraction antibody of anti-Human IL-1a has been tested for use in Neutralizations,ELISA, Immunohistochemistry, Flow Cytometry and Immunoblotting. It recognizes the17,000 MW mature IL-1a . Reactivity in other immunoassays is unknown. Recommended Dilutions: This product has been assayed by immunoblot using HRPGoat-anti-Rabbit IgG [H&L] and TMB as a substrate. A working dilution range of 1: 100 to1: 200 is suggested for this application to detect IL-1a from supernatants or lysates of 2 x10e6 endotoxin-stimulated human peripheral blood mononuclear cells (PBMC). PBMC arestimulated for 24 hours with 1% (v/v) human serum plus 10 ng/mL E. coli LPS. This producthas been assayed by Immunohistochemistry. A dilution range of 1: 200 is suggested for thisimmunoassay. Either paraffin fixation or cryofixation can be used forImmunohistochemistry using a dilution of 1: 200 for staining of intracellular IL-1a . This

Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)

Purification: Purified

Buffer: 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 containing0.01% (w/v) Sodium Azide as preservative.

Storage: Store the antibody (undiluted) at 2-8°C for one month or (in aliquots) at -20°C for longer. Dilute only prior to immediate use. Avoid cycles of freezing and thawing. Shelf life: One year from despatch.

Restrictions: For Research Use only

Publications

March, Mosley, Larsen et al.: "Cloning, sequence and expression of two distinct human interleukin-1 complementary DNAs." in: Nature, Vol. 315, Issue 6021, pp. 641-7, 1985 (PubMed).