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MAP3K5 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 5) (AA 76-87)

Details for Product anti-MAP3K5 Antibody No. ABIN116930, Supplier: Login to see
Antigen
  • 7420452D20Rik
  • ASK
  • ASK1
  • MAPKKK5
  • Mekk5
  • mitogen-activated protein kinase kinase kinase 5
  • MUD21.11
  • MUD21_11
  • MEKK5
  • MAP3K5
Alternatives
anti-Human MAP3K5 antibody for Western Blotting
Epitope
AA 76-87, pSer83
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13
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Reactivity
Human
371
138
78
4
1
Host
Rabbit
357
22
Clonality
Polyclonal
Conjugate
This MAP3K5 antibody is un-conjugated
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11
6
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Application
Western Blotting (WB)
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57
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39
23
11
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6
2
1
1
1
Supplier
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Immunogen This purified antibody was prepared from rabbit serum after repeated immunizations with a KLH conjugated peptide corresponding to amino acids 76-87 of human ASK-1 protein.
Specificity This product is an IgG fraction antibody purified from antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. No reaction was observed with ASK-1 from mouse sources. Reactivity with the kinase from other sources has not been determined.
Purification Multi-step process.
Alternative Name MEKK5 / ASK1 (MAP3K5 Antibody Abstract)
Background ASK-1 (apoptosis signal-regulating kinase 1- also referred to as MEK Kinase-5 or MAPKKK5) is a novel serine/threonine MAP kinase kinase kinase (MAPKKK) component of the mitogen -activated protein (MAP) cascade that is activated in response to extracellular stimuli by cytokines, growth factors and environmental stresses and other factors. Overexpression of ASK-1 induces apoptotic cell death. ASK-1 is expressed in a variety of human and mouse tissues. The overall amino acid sequence identity between the mouse and human ASK1 is 91.9 % . ASK-1 interacts with CDKN1A (also known as p21, WAF1, CIP1). Please refer to the reference list at the end of this document for further information.Synonyms: Apoptosis signal-regulating kinase 1, MAP3K5, MAPK/ERK kinase kinase 5, MAPKKK5, Mitogen-activated protein kinase kinase kinase 5
Gene ID 4217
NCBI Accession NP_005914
UniProt Q99683
Pathways MAPK Signaling
Application Notes This phospho specific polyclonal antibody reacts human pS83 ASK1 and shows minimalreactivity by Western blot, ELISA and competitive ELISA with non-phosphorylated ASK1. Although not tested, this antibody is likely functional in RIA, Immunohistochemistry andImmunoprecipitation. Recommended Dilutions: For immunoblotting a 1/1,000 dilution is recommended. A 155 kDa band corresponding to human ASK-1 is detected. Whole cell lysates from SW1353 canbe used as a positive control. For ELISA a 1/5,000-1/10,000 dilution is recommended.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 2.0 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % sodium azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Dilute only prior to immediate use . Avoid cycles of freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at -20 °C.
Supplier Images
 image for anti-MAP3K5 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 5) (AA 76-87) (ABIN116930) Immunoblot of anti-pS83 ASK1 antibodies shows specificity for phosphorylated human AS...
 image for anti-MAP3K5 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 5) (AA 76-87) (ABIN116930) ELISA results of purified polyclonal anti-pS83 ASK-1 (aa 76-87) antibody tested again...
Background publications Morita, Saitoh, Tobiume et al.: "Negative feedback regulation of ASK1 by protein phosphatase 5 (PP5) in response to oxidative stress." in: The EMBO journal, Vol. 20, Issue 21, pp. 6028-36, 2001 (PubMed).

Matsuzawa, Ichijo: "Molecular mechanisms of the decision between life and death: regulation of apoptosis by apoptosis signal-regulating kinase 1." in: Journal of biochemistry, Vol. 130, Issue 1, pp. 1-8, 2001 (PubMed).

Zou, Tsutsui, Ray et al.: "The cell cycle-regulatory CDC25A phosphatase inhibits apoptosis signal-regulating kinase 1." in: Molecular and cellular biology, Vol. 21, Issue 14, pp. 4818-28, 2001 (PubMed).

Geleziunas, Xu, Takeda et al.: "HIV-1 Nef inhibits ASK1-dependent death signalling providing a potential mechanism for protecting the infected host cell." in: Nature, Vol. 410, Issue 6830, pp. 834-8, 2001 (PubMed).

Cho, Lee, Park et al.: "Glutathione S-transferase mu modulates the stress-activated signals by suppressing apoptosis signal-regulating kinase 1." in: The Journal of biological chemistry, Vol. 276, Issue 16, pp. 12749-55, 2001 (PubMed).

Tobiume, Matsuzawa, Takahashi et al.: "ASK1 is required for sustained activations of JNK/p38 MAP kinases and apoptosis." in: EMBO reports, Vol. 2, Issue 3, pp. 222-8, 2001 (PubMed).

Noguchi, Kokubu, Kitanaka et al.: "ASK1-signaling promotes c-Myc protein stability during apoptosis." in: Biochemical and biophysical research communications, Vol. 281, Issue 5, pp. 1313-20, 2001 (PubMed).

Sayama, Hanakawa, Shirakata et al.: "Apoptosis signal-regulating kinase 1 (ASK1) is an intracellular inducer of keratinocyte differentiation." in: The Journal of biological chemistry, Vol. 276, Issue 2, pp. 999-1004, 2001 (PubMed).

Mochida, Takeda, Saitoh et al.: "ASK1 inhibits interleukin-1-induced NF-kappa B activity through disruption of TRAF6-TAK1 interaction." in: The Journal of biological chemistry, Vol. 275, Issue 42, pp. 32747-52, 2000 (PubMed).

Hatai, Matsuzawa, Inoshita et al.: "Execution of apoptosis signal-regulating kinase 1 (ASK1)-induced apoptosis by the mitochondria-dependent caspase activation." in: The Journal of biological chemistry, Vol. 275, Issue 34, pp. 26576-81, 2000 (PubMed).

Takeda, Hatai, Hamazaki et al.: "Apoptosis signal-regulating kinase 1 (ASK1) induces neuronal differentiation and survival of PC12 cells." in: The Journal of biological chemistry, Vol. 275, Issue 13, pp. 9805-13, 2000 (PubMed).

Liu, Nishitoh, Ichijo et al.: "Activation of apoptosis signal-regulating kinase 1 (ASK1) by tumor necrosis factor receptor-associated factor 2 requires prior dissociation of the ASK1 inhibitor thioredoxin." in: Molecular and cellular biology, Vol. 20, Issue 6, pp. 2198-208, 2000 (PubMed).

Yasuda, Inoue, Hirabayashi et al.: "Triggering of neuronal cell death by accumulation of activated SEK1 on nuclear polyglutamine aggregations in PML bodies." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 4, Issue 12, pp. 743-56, 2000 (PubMed).

Kanamoto, Mota, Takeda et al.: "Role of apoptosis signal-regulating kinase in regulation of the c-Jun N-terminal kinase pathway and apoptosis in sympathetic neurons." in: Molecular and cellular biology, Vol. 20, Issue 1, pp. 196-204, 2000 (PubMed).