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LEFTY2 antibody

LEFTY2 Reactivity: Human, Mouse, Rat WB, IHC (p), EIA Host: Mouse Monoclonal 7C5G1H6H10 unconjugated
Catalog No. ABIN116931
  • Target See all LEFTY2 Antibodies
    LEFTY2 (Left-Right Determination Factor 2 (LEFTY2))
    Reactivity
    • 30
    • 13
    • 10
    • 3
    • 1
    • 1
    • 1
    Human, Mouse, Rat
    Host
    • 33
    • 5
    • 2
    • 1
    Mouse
    Clonality
    • 36
    • 5
    Monoclonal
    Conjugate
    • 26
    • 9
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This LEFTY2 antibody is un-conjugated
    Application
    • 33
    • 15
    • 14
    • 5
    • 5
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
    Specificity
    This protein A purified mouse monoclonal antibody reacts with a 30 kDa protein corresponding to human LEFTY. No reactivity occurs against 6X HIS tag. Significant sequence homology exists between the human and mouse forms of LEFTY. Cross reactivity is expected with mouse and rat forms of the protein. Reactivity with other sources has not been determined.
    Purification
    Protein A chromatography.
    Immunogen
    A BALB/c mouse was immunized with a recombinant form of 6X HIS tagged human LEFTY.
    Clone
    7C5G1H6H10
    Isotype
    IgG2a
    Top Product
    Discover our top product LEFTY2 Primary Antibody
  • Application Notes
    This antibody is suitable for ELISA (1/5,000) and Western blotting (1/2,000). The antibodymay be used for other applications, such as RIA, Immunohistochemistry orImmunoprecipitation, but specific reaction conditions have not been developed.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL (by UV absorbance at 280 nm)
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % sodium azide as preservative.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Target
    LEFTY2 (Left-Right Determination Factor 2 (LEFTY2))
    Alternative Name
    LEFTY2 (LEFTY2 Products)
    Synonyms
    EBAF antibody, LEFTA antibody, LEFTYA antibody, TGFB4 antibody, AI450052 antibody, Ebaf antibody, Leftb antibody, Stra3 antibody, Tgfb4 antibody, lefty antibody, lefty-1 antibody, atv2 antibody, cb720 antibody, fe38b03 antibody, wu:fe38b03 antibody, 6030463A22Rik antibody, AV214969 antibody, Lefta antibody, Ebaf2 antibody, left-right determination factor 2 antibody, left right determination factor 1 antibody, lefty2 antibody, Left-right determination factor 2 antibody, left-right determination factor 2-like antibody, LEFTY2 antibody, Lefty1 antibody, lft2 antibody, Lefty2 antibody, LOC699676 antibody, LOC100232580 antibody
    Background
    During vertebrate embryogenesis, a left-right axis is established. Secreted growth factors of the TGF-beta family, including gene products derived from nodal, lefty-1 and lefty-2, play crucial roles in establishing left-right asymmetries. TGF-beta (Transforming growth factor-beta) is a pleiotropic cytokine that regulates growth and differentiation of diverse types of cells. TGF-beta actions are directed by ligand-induced activation of TGF-beta receptors. Complexes formed move into the nucleus, where they act as components of a transcriptional complex. Lefty, a novel member of the TGF-beta superfamily, inhibits TGF-beta signaling. Lefty acts to inhibit phosphorylation of Smad2 following activation of the TGF-beta receptor. Lefty also inhibits events downstream from R-Smad phosphorylation. Lefty provides a repressed state of TGF-beta-responsive genes. The Lefty family is comprised of Lefty 1 and Lefty 2 in mouse, and Lefty A and Lefty B in humans. Members of the TGF-beta superfamily require processing for their activation. Cleavage is therefore an essential step for Lefty activation. Lefty is synthesized as a large inactive precursor (42 Kda) that must be endoproteolytically processed to release the bioactive polypeptide (28 kDa and 34 kDa forms). The 28 kDa form induces MAPK activity.Synonyms: EBAF, Endometrial bleeding-associated factor, LEFTA, LEFTY2, LEFTYA, Left-right determination factor 2, Left-right determination factor A, Protein lefty-2, Protein lefty-A, TGF-beta-4, TGFB4, Transforming growth factor beta-4
    Gene ID
    7044
    NCBI Accession
    NP_003231
    UniProt
    O00292
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