Left-Right Determination Factor 2 (LEFTY2) antibody

Details for Product No. ABIN116931
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Synonyms atv2, cb720, fe38b03, wu:fe38b03, LEFTY1, AI450052, Ebaf, Leftb, Stra3, Tgfb4, lefty, lefty-1, EBAF, LEFTA, LEFTYA, TGFB4, 6030463A22Rik, AV214969, Lefta, Ebaf2
Human, Mouse (Murine), Rat (Rattus)
(38), (24), (21), (1), (1), (1)
(38), (8), (2), (1)
Clonality (Clone)
Monoclonal ()
(7), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(36), (23), (14), (10), (10), (7), (6), (4), (2), (2), (1)
Pubmed 4 references available
Quantity 0.1 mg
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Catalog No. ABIN116931
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Immunogen A BALB/c mouse was immunized with a recombinant form of 6X HIS tagged human LEFTY.
Clone 7C5G1H6H10
Isotype IgG2a
Specificity This protein A purified mouse monoclonal antibody reacts with a 30 kDa protein corresponding to human LEFTY. No reactivity occurs against 6X HIS tag. Significant sequence homology exists between the human and mouse forms of LEFTY. Cross reactivity is expected with mouse and rat forms of the protein. Reactivity with other sources has not been determined.
Purification Protein A chromatography.
Alternative Name LEFTY2
Background During vertebrate embryogenesis, a left-right axis is established. Secreted growth factors of the TGF-beta family, including gene products derived from nodal, lefty-1 and lefty-2, play crucial roles in establishing left-right asymmetries. TGF-beta (Transforming growth factor-beta) is a pleiotropic cytokine that regulates growth and differentiation of diverse types of cells. TGF-beta actions are directed by ligand-induced activation of TGF-beta receptors. Complexes formed move into the nucleus, where they act as components of a transcriptional complex. Lefty, a novel member of the TGF-beta superfamily, inhibits TGF-beta signaling. Lefty acts to inhibit phosphorylation of Smad2 following activation of the TGF-beta receptor. Lefty also inhibits events downstream from R-Smad phosphorylation. Lefty provides a repressed state of TGF-beta-responsive genes. The Lefty family is comprised of Lefty 1 and Lefty 2 in mouse, and Lefty A and Lefty B in humans. Members of the TGF-beta superfamily require processing for their activation. Cleavage is therefore an essential step for Lefty activation. Lefty is synthesized as a large inactive precursor (42 Kda) that must be endoproteolytically processed to release the bioactive polypeptide (28 kDa and 34 kDa forms). The 28 kDa form induces MAPK activity.Synonyms: EBAF, Endometrial bleeding-associated factor, LEFTA, LEFTY2, LEFTYA, Left-right determination factor 2, Left-right determination factor A, Protein lefty-2, Protein lefty-A, TGF-beta-4, TGFB4, Transforming growth factor beta-4
Gene ID 7044
NCBI Accession NP_003231
UniProt O00292
Research Area Cancer, Growth Factors
Application Notes This antibody is suitable for ELISA (1/5,000) and Western blotting (1/2,000). The antibodymay be used for other applications, such as RIA, Immunohistochemistry orImmunoprecipitation, but specific reaction conditions have not been developed.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % sodium azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
anti-Left-Right Determination Factor 2 (LEFTY2) antibody Mab anti-Human LEFTY antibody (clone 7C5G1H6H10) is shown to detect by western blot partially purified recombinant 6X His tagged human LEFTY. Detection occurs after 1.0 ug of protein is loaded in each lane. The blot was incubated with a 1:2,000 dilution of Mab anti-Human LEFTY at room temperature for 30 min followed by detection using IRDye™800 labeled Goat-a-Mouse IgG [H&L] (610-132-121) diluted 1:1,000. Lane 1 contains an unrelated 6X His tagged protein and shows that the antibody does not recognize the epitope tag. Lane 2 contains partially purified recombinant human LEFTY. The antibody may be used to detect endogenous human LEFTY. IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
Background publications Ulloa, Tabibzadeh: "Lefty inhibits receptor-regulated Smad phosphorylation induced by the activated transforming growth factor-beta receptor." in: The Journal of biological chemistry, Vol. 276, Issue 24, pp. 21397-404, 2001 (PubMed).

Tsukui, Capdevila, Tamura et al.: "Multiple left-right asymmetry defects in Shh(-/-) mutant mice unveil a convergence of the shh and retinoic acid pathways in the control of Lefty-1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 20, pp. 11376-81, 1999 (PubMed).

Nonaka, Tanaka, Okada et al.: "Randomization of left-right asymmetry due to loss of nodal cilia generating leftward flow of extraembryonic fluid in mice lacking KIF3B motor protein." in: Cell, Vol. 95, Issue 6, pp. 829-37, 1999 (PubMed).

Meno, Shimono, Saijoh et al.: "lefty-1 is required for left-right determination as a regulator of lefty-2 and nodal." in: Cell, Vol. 94, Issue 3, pp. 287-97, 1998 (PubMed).

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