CAMP Responsive Element Binding Protein 1 (CREB1) (pSer133) antibody

Details for Product No. ABIN116949
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Antigen
Synonyms CREB, Creb, Creb-1, AV083133, 2310001E10Rik, 3526402H21Rik, creb, creb1, zgc:55598, CREB1, Creb1
Epitope
pSer133
(72), (36), (26), (19), (14), (13), (9), (7), (5), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(329), (241), (226), (75), (68), (67), (44), (9), (6), (5), (4), (1)
Host
Rabbit
(293), (50), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(8), (7), (6), (6), (6), (6), (6), (6), (6), (6), (6)
Application
ELISA, Immunoprecipitation (IP), Western Blotting (WB)
(251), (131), (131), (73), (60), (41), (31), (24), (8), (2), (2), (1), (1), (1), (1)
Pubmed 7 references available
Catalog no. ABIN116949
Quantity 0.1 mL
Price
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Immunogen CREB phospho peptide corresponding to a region of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Sequence Q-K-R-R-E-I-L-S-R-R-P-pS-Y-R-K-I-L-N-D-L-S-S-D-A-P corresponding to aa 122-147
Specificity This phospho specific polyclonal antibody reacts with phosphorylated human CREB at pS133 and shows minimal reactivity by western blot with non-phosphorylated CREB and minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. This product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first sequentially pre-adsorbed against a control E.coli lysate, nickel-purified recombinant CREB and the non-phosphorylated form of the immunizing peptide. The resultant depleted antiserum was then purified against the phosphorylated form of the immunizing peptide. This phospho specific polyclonal antibody is specific for phosphorylated pS133 of human CREB. Reactivity with non-phosphorylated CREB is minimal. This antibody does show cross reactivity with pS133 phosphorylated CREB from mouse and rat.
Purification Affinity Purified
Alternative Name CREB1
Background The CREB (Cyclic AMP-response-element-binding-protein 1) gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element (CRE element TGANNTCA), an octameric palindrome. Phosphorylation by cAMPdependent protein kinase (PKA) at serine-119 is required for interaction with DNA and phosphorylation at serine-133 allows CREB to interact with CBP (CREB binding protein) leading to interaction with RNA polymerase II. Alternate splicing of this gene results in two transcript variants encoding different isoforms.
Alternate names: CREB-1, Cyclic AMP-responsive element-binding protein 1, cAMP-responsive element- binding protein 1
Gene ID 1385
NCBI Accession NP_004370.1
UniProt P16220
Application Notes Western Blot: 1: 1,000. ELISA. Immunoprecipitation. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.064 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.1% (w/v) Sodium Azide
Preservative Sodium azide
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Supplier Images
anti-CAMP Responsive Element Binding Protein 1 (CREB1) (pSer133) antibody Anti-CREB pS133 was used to detect phosphorylated CREB by immunoblot. Recombinant His-tagged human CREB was produced in E.coli and purified by metal affinity chromatography. An aliquot of purified CREB was phosphorylated in-vitro using Protein Kinase A and ATP. Immunoblot of control (-) and in-vitro phosphorylated CREB (+) was used to show that the antibody reacts specifically with the phosphorylated form. Pan reactive CREB (#ABIN116948) reacts equally with both nonphosphorylated and phosphorylated CREB (not shown). Detection occurs using a 1:1,000 dilution of antibody followed by 1:5,000 dilution of HRP Goat-a-Rabbit IgG with visualization via ECL. Film exposure approximately 1’. Other detection systems will yield similar results. Data contributed by Boss, J., Emory University School of Medicine, personal communication.
anti-CAMP Responsive Element Binding Protein 1 (CREB1) (pSer133) antibody (2) Anti-CREB pS133 was used to detect phosphorylated CREB by immunoblot. Recombinant His-tagged human CREB was produced in E.coli and purified by metal affinity chromatography. An aliquot of purified CREB was phosphorylated in-vitro using Protein Kinase A and ATP. Immunoblot of indicated amounts of control (-) and in-vitro phosphorylated CREB (P) were loaded to show that the antibody reacts specifically with the phosphorylated form. Blots were blocked in 5% milk in TBS+0.1% Tween-20 (TBST-M) overnight at 4°C. Detection occurs using a 1:1,000 dilution of antibody diluted in TBST-M and incubated at room temperature with rocking for 1 hour. Blots were rinsed 6X with TBST and incubated with goat anti-rabbit-HRP at 1:5000 in TBST-M at room temperature for 45 min. Blots were again rinsed 6X with TBST and then processed using ECL reagent (Amersham) according to manufacturer's instructions. Exposure time: 1 min using Kodak Biomax MR film. Data contributed by Dr. R. Screaton, The Salk Institute for Biological Studies, personal communication.
Background publications Dwarki, Montminy, Verma: "Both the basic region and the 'leucine zipper' domain of the cyclic AMP response element binding (CREB) protein are essential for transcriptional activation." in: The EMBO journal, Vol. 9, Issue 1, pp. 225-32, 1990 (PubMed).

Lee, Yun, Hoeffler et al.: "Cyclic-AMP-responsive transcriptional activation of CREB-327 involves interdependent phosphorylated subdomains." in: The EMBO journal, Vol. 9, Issue 13, pp. 4455-65, 1991 (PubMed).

Barton, Muthusamy, Chanyangam et al.: "Defective thymocyte proliferation and IL-2 production in transgenic mice expressing a dominant-negative form of CREB." in: Nature, Vol. 379, Issue 6560, pp. 81-5, 1996 (PubMed).

Kwok, Laurance, Lundblad et al.: "Control of cAMP-regulated enhancers by the viral transactivator Tax through CREB and the co-activator CBP." in: Nature, Vol. 380, Issue 6575, pp. 642-6, 1996 (PubMed).

Moreno, Beresford, Louis-Plence et al.: "CREB regulates MHC class II expression in a CIITA-dependent manner." in: Immunity, Vol. 10, Issue 2, pp. 143-51, 1999 (PubMed).

Arthur, Cohen: "MSK1 is required for CREB phosphorylation in response to mitogens in mouse embryonic stem cells." in: FEBS letters, Vol. 482, Issue 1-2, pp. 44-8, 2000 (PubMed).

Kim, Lu, Quinn: "Distinct cAMP response element-binding protein (CREB) domains stimulate different steps in a concerted mechanism of transcription activation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 21, pp. 11292-6, 2000 (PubMed).

Hosts (293), (50), (1)
Reactivities (329), (241), (226), (75), (68), (67), (44), (9), (6), (5), (4), (1)
Applications (251), (131), (131), (73), (60), (41), (31), (24), (8), (2), (2), (1), (1), (1), (1)
Conjugates (8), (7), (6), (6), (6), (6), (6), (6), (6), (6), (6)
Epitopes (72), (36), (26), (19), (14), (13), (9), (7), (5), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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