ATG12 antibody

Catalog No. ABIN116953

Product Details anti-ATG12 Antibody

Target: ATG12 (Autophagy Related 12 (ATG12))

Reactivity: Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG12 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.

Cross-Reactivity (Details): Species reactivity (tested):S. cerevisiae, Yeast

Purification: A multi-step process which includes Delipidation, Salt Fractionation and Ion exchange chromatography followed by extensive dialysis against the buffer stated below.

Immunogen: This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant yeast APG12 protein.

Application Details

Application Notes: This purified polyclonal antibody reacts with yeast APG12 by Western blot and ELISA. This antibody using the specified conditions may recognize other prominent intrinsicbands (UBLs or conjugates).
Other intrinsic bands are readily detectable at lower dilutions. A 22.1 kDa band corresponding to yeast APG12 is detected. Most yeast cell lysates can be used as a positive control without induction or stimulation. Recommended Dilutions: ELISA: 1/1,000-1/5,000. Western Blot: 1/200. Although not tested, this antibody is likely functional in Immunohistochemistry andImmunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.1 mL of deionized water or equivalent.

Concentration: 5.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.

Target Details for ATG12

Target: ATG12 (Autophagy Related 12 (ATG12))

Alternative Name: ATG12 / APG12 (ATG12 Products)

Background: Ubiquitin-like proteins fall into two classes: the first class, ubiquitin-like modifiers (UBLs) function as modifiers in a manner analogous to that of ubiquitin. Examples of UBLs are SUMO, Rub1 (also called Nedd8), Apg8 and Apg12. Proteins of the second class include parkin, RAD23 and DSK2, are designated ubiquitin-domain proteins (UDPs). These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other. In contrast to UBLs, UDPs are not conjugated to other proteins. In yeast, autophagy, the delivery of cytoplasmic components to the lysosome/vacuole for degradation, requires a ubiquitin-like protein conjugation system, in which Apg12 is covalently bound to Apg12-Apg5 and Apg16.Synonyms: APG12-like, APG12L, Autophagy-related protein 12, Ubiquitin-like protein ATG12

Gene ID: 852518

NCBI Accession: NP_009776

UniProt: P38316

Pathways: Autophagy