ATG12 antibody (N-Term)

Catalog No. ABIN6254241

Cited in 1 publication. The Goat Polyclonal anti-ATG12 antibody (ABIN6254241) specifically detects ATG12 in WB and IF. The antibody is reactive with Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Monkey, Chicken, Rabbit, Pig, Cat, Donkey, Goat, Hamster, Sheep and Avian samples.

Quick Overview for ATG12 antibody (N-Term) (ABIN6254241)

Target: ATG12 (Autophagy Related 12 (ATG12))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Monkey, Chicken, Rabbit, Pig, Cat, Donkey, Goat, Hamster, Sheep, Avian

Host: Goat

Clonality: Polyclonal

Conjugate: This ATG12 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-ATG12 Antibody

Binding Specificity: N-Term

Purpose: Anti-ATG12

Sequence: AEEPQSVLQL PTSIAAGGEG LTDVSPETTT PEPPSSAAVS PGTEEPAGD

Specificity: Detects GFP-ATG12 in transfected cells by Western blot.

Characteristics: Autophagy related 12

Purification: Epitope affinity purified

Immunogen: Purified recombinant peptide within residues 65 aa to the N-terminus of human ATG12 produced in E. coli.

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:2,000, IF:1:50-1:250, IHC-P:1:200-1:1,000, IHC-F:1:200-1:1,000

Restrictions: For Research Use only

Handling

Buffer: PBS, 20 % glycerol and 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody solution should be gently mixed before use.

Storage: 4 °C,-20 °C

Storage Comment: For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.

References for anti-ATG12 antibody (ABIN6254241)

Kharaziha, Panaretakis: "Dynamics of Atg5-Atg12-Atg16L1 Aggregation and Deaggregation." in: Methods in enzymology, Vol. 587, pp. 247-255, (2017) (PubMed).

Target Details for ATG12

Target: ATG12 (Autophagy Related 12 (ATG12))

Alternative Name: ATG12

Background:

Goat polyclonal antibody to ATG12 - autophagosome marker. Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy.

APG12, Apg12 (autophagy, yeast) homolog, APG12L, FBR93, HAPG12, autophagy protein 12, autophagy-related protein 12 antibody.

Pathways: Autophagy