B-Cell Linker antibody (N-Term) (Alexa Fluor 488)

Catalog No. ABIN1176913

Product Details anti-B-Cell Linker Antibody (Alexa Fluor 488)

Target: B-Cell Linker (BLNK)

Binding Specificity: N-Term

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This B-Cell Linker antibody is conjugated to Alexa Fluor 488

Application: Intracellular Staining (ICS)

Brand: BD Phosflow™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human N-terminal BLNK Recombinant Protein

Clone: 2B11

Isotype: IgG2a kappa

Application Details

Application Notes: This antibody conjugate is suitable for intracellular staining of human whole blood (using BD™ Phosflow Lyse/Fix Buffer) and peripheral blood mononuclear cells (using BD Cytofix™ Fixation Buffer). Any of the three BD™ Phosflow permeabilization buffers may be used.

Sample Volume: 20 μL

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed.

References for anti-B-Cell Linker antibody (Alexa Fluor 488) (ABIN1176913)

Li, Martin, Oliver, Kearney, Carter: "Antigen receptor proximal signaling in splenic B-2 cell subsets." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 5, pp. 3122-9, (2001) (PubMed).

Target Details for B-Cell Linker

Target: B-Cell Linker (BLNK)

Alternative Name: BLNK (BLNK Products)

Background: B cell activation is initiated by crosslinking the B cell receptor, which leads to activation of non-receptor protein tyrosine kinases (PTK), including Btk, Syk, and three Src kinases, Fyn, Lyn, and Blk. Activated PTKs then phosphorylate multiple cellular proteins involved in B lymphocyte signaling. Syk is responsible for the tyrosine phosphorylation of B cell linker protein (BLNK), a member of the SLP-76 family of adapter proteins. Phosphorylation of human BLNK at tyrosines 84, 178, and 189 (Y84, Y178, and Y189) creates docking sites for PLCg2, leading to the activation of downstream signaling pathways. The 2B11 monoclonal antibody recognizes BLNK, regardless of phosphorylation status. A fusion protein representing amino acids 4-205 of human BLNK was used as the immunogen. BLNK is expressed as two phosphoproteins migrating at 68 and 70 kDa in SDS/PAGE that represent alternatively spliced forms of human BLNK.
Synonyms: SLP-65, BASH, BCA

Pathways: BCR Signaling