CD48 antibody

Catalog No. ABIN1177003

Product Details anti-CD48 Antibody

Target: CD48

Reactivity: Mouse

Host: Armenian Hamster

Clonality: Monoclonal

Conjugate: This CD48 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Functional Studies (Func), Blocking Reagent (BR)

Brand: BD Pharmingen™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogen: Mouse T lymphoma MBL-2

Clone: HM48-1

Isotype: IgG1 lambda

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-CD48 antibody (ABIN1177003)

Latchman, McKay, Reiser: "Identification of the 2B4 molecule as a counter-receptor for CD48." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 11, pp. 5809-12, (1998) (PubMed).

Qin, Chavin, Lin, Yagita, Bromberg: "Anti-CD2 receptor and anti-CD2 ligand (CD48) antibodies synergize to prolong allograft survival." in: The Journal of experimental medicine, Vol. 179, Issue 1, pp. 341-6, (1994) (PubMed).

Kato, Koyanagi, Okada, Takanashi, Wong, Williams, Okumura, Yagita: "CD48 is a counter-receptor for mouse CD2 and is involved in T cell activation." in: The Journal of experimental medicine, Vol. 176, Issue 5, pp. 1241-9, (1992) (PubMed).

Target Details for CD48

Target: CD48

Alternative Name: CD48 (BCM1) (CD48 Products)

Background: The HM48-1 monoclonal antibody specifically binds to CD48 (previously known as BCM1 in mice, Blast-1 in human, and OX-45 in the rat), a GPI-anchored member of the Ig superfamily. It is widely distributed on leukocytes, but not on non-hematopoietic cells, and its ligands include CD2 (LFA-2) and CD244 (2B4 antigen). The HM48-1 mAb blocks binding of soluble CD2 to CD48-bearing cells, blocks the interaction of CD2 and CD244, inhibits spleen cell proliferative responses to mitogens, augments the proliferative response of spleen cells when cross-linked with anti-CD3e mAbs, and inhibits priming of CTL in vitro. In vivo administration of HM48-1 antibody can prolong survival of cardiac allografts, an effect which is greatly enhanced by the addition of anti-CD2 mAb 12-15. This hamster mAb to a mouse leukocyte antigen does not cross-react with rat leukocytes.
Synonyms: BLAST, BLAST-1, BCM1, HM48-1, MEM-102, Sgp-60, SLAMF2