B-Cell Linker antibody (pTyr84) (PE)
(1 reference)-
- Target See all B-Cell Linker (BLNK) Antibodies
- B-Cell Linker (BLNK)
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Binding Specificity
- pTyr84
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Reactivity
- Human, Mouse
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This B-Cell Linker antibody is conjugated to PE
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Application
- Intracellular Staining (ICS)
- Brand
- BD Phosflow™
- Purification
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Immunogen
- Phosphorylated Human BLNK Peptide
- Clone
- J117-1278
- Isotype
- IgG2b kappa
- Top Product
- Discover our top product BLNK Primary Antibody
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anti-B-Cell Linker (BLNK) antibody
BLNK Reactivity: Human WB, ELISA, IHC, FACS, ICC Host: Mouse Monoclonal 5G9 unconjugated
anti-B-Cell Linker (BLNK) (AA 65-314) antibodyBLNK Reactivity: Human WB, IHC, ICC, IP Host: Rabbit Polyclonal unconjugated
anti-B-Cell Linker (BLNK) (C-Term) antibodyVerified BLNK Reactivity: Human WB, ELISA, FACS, IF Host: Goat Polyclonal unconjugated
anti-B-Cell Linker (BLNK) (AA 301-400) antibodyBLNK Reactivity: Human WB, ELISA, PLA Host: Mouse Monoclonal 1D7 unconjugated
anti-B-Cell Linker (BLNK) (AA 41-337) antibodyBLNK Reactivity: Human WB, ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated
anti-B-Cell Linker (BLNK) (pTyr96) antibodyBLNK Reactivity: Human WB, ELISA, IF (p), IHC (p), IF (cc), IHC (fro) Host: Rabbit Polyclonal unconjugated
anti-B-Cell Linker (BLNK) (pTyr96) antibodyBLNK Reactivity: Human, Mouse WB, ELISA, IHC, ICC, IF Host: Rabbit Polyclonal unconjugated
anti-B-Cell Linker (BLNK) (N-Term) antibodyBLNK Reactivity: Human, Mouse WB, ELISA, IHC, ICC, IF Host: Rabbit Polyclonal unconjugated
anti-B-Cell Linker (BLNK) (AA 150-178) antibodyBLNK Reactivity: Human WB, FACS, IHC (p) Host: Rabbit Polyclonal RB18883 unconjugated
anti-B-Cell Linker (BLNK) (pTyr84) antibodyBLNK Reactivity: Human WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated
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- Sample Volume
- 20 μL
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
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: "Impaired B-cell development at the pre-BII-cell stage in galectin-1-deficient mice due to inefficient pre-BII/stromal cell interactions." in: Blood, Vol. 113, Issue 23, pp. 5878-86, (2009) (PubMed).
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: "Impaired B-cell development at the pre-BII-cell stage in galectin-1-deficient mice due to inefficient pre-BII/stromal cell interactions." in: Blood, Vol. 113, Issue 23, pp. 5878-86, (2009) (PubMed).
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- Target
- B-Cell Linker (BLNK)
- Alternative Name
- BLNK (BLNK Products)
- Background
- B cell activation is initiated by crosslinking the B cell receptor, which leads to activation of non-receptor protein tyrosine kinases (PTK), including Btk, Syk, and three Src kinases, Fyn, Lyn, and Blk. Activated PTKs then phosphorylate multiple cellular proteins involved in B lymphocyte signaling. Syk is responsible for the tyrosine phosphorylation of B cell linker protein (BLNK), a member of the SLP-76 family of adapter proteins. Phosphorylation of human BLNK at tyrosines 84, 178, and 189 (Y84, Y178, and Y189) creates docking sites for PLCg2, leading to the activation of downstream signaling pathways. The J117-1278 monoclonal antibody recognizes the phosphorylated Y84 of BLNK.
- Pathways
- BCR Signaling
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