ELK1 antibody (pSer383) (Alexa Fluor 647)

Catalog No. ABIN1177054

Product Details anti-ELK1 Antibody (Alexa Fluor 647)

Target: ELK1 (ELK1, Member of ETS Oncogene Family (ELK1))

Binding Specificity: pSer383

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This ELK1 antibody is conjugated to Alexa Fluor 647

Application: Intracellular Staining (ICS)

Brand: BD Phosflow™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Phosphorylated Human Elk-1 Peptide

Clone: M21-1721

Isotype: IgG1 kappa

Application Details

Application Notes: This antibody conjugate is suitable for intracellular staining of human peripheral blood mononuclear cells using BD Cytofix™ Fixation Buffer. Any of the three BD Phosflow™ permeabilization buffers may be used.

Sample Volume: 20 μL

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.

References for anti-ELK1 antibody (Alexa Fluor 647) (ABIN1177054)

Bardwell, Abdollahi, Bardwell: "Docking sites on mitogen-activated protein kinase (MAPK) kinases, MAPK phosphatases and the Elk-1 transcription factor compete for MAPK binding and are crucial for enzymic activity." in: The Biochemical journal, Vol. 370, Issue Pt 3, pp. 1077-85, (2003) (PubMed).

Espanel, Wälchli, Rückle, Harrenga, Huguenin-Reggiani, Hooft van Huijsduijnen: "Mapping of synergistic components of weakly interacting protein-protein motifs using arrays of paired peptides." in: The Journal of biological chemistry, Vol. 278, Issue 17, pp. 15162-7, (2003) (PubMed).

Tian, Karin: "Stimulation of Elk1 transcriptional activity by mitogen-activated protein kinases is negatively regulated by protein phosphatase 2B (calcineurin)." in: The Journal of biological chemistry, Vol. 274, Issue 21, pp. 15173-80, (1999) (PubMed).

Sugimoto, Stewart, Guan: "The calcium/calmodulin-dependent protein phosphatase calcineurin is the major Elk-1 phosphatase." in: The Journal of biological chemistry, Vol. 272, Issue 47, pp. 29415-8, (1997) (PubMed).

Cavigelli, Dolfi, Claret, Karin: "Induction of c-fos expression through JNK-mediated TCF/Elk-1 phosphorylation." in: The EMBO journal, Vol. 14, Issue 23, pp. 5957-64, (1996) (PubMed).

Target Details for ELK1

Target: ELK1 (ELK1, Member of ETS Oncogene Family (ELK1))

Alternative Name: Elk-1 (ELK1 Products)

Background: Elk-1 is a 62-kDa member of the Ets oncogene family of transcription factors and a member of the subfamily of ternary complex factors (TCF). Ets proteins mediate a variety of gene activities in response to serum and growth factors. Proteins in the TCF subfamily form a ternary complex by binding to the Serum Response Element (SRE) in conjunction with a dimer of Serum Response Factors (SRF). Elk-1 is phosphorylated by mitogen-activated protein (MAP) kinase pathways in vivo at a cluster of S/T motifs at its carboxy-terminus. Phosphorylation at these sites, particularly at serine 383 (S383), is critical for Elk-1 transcriptional activation. Studies have shown that Elk-1 is a direct target of activated MAP kinase, and that it is also a target of the stress-activated kinase SAPK/JNK. The M21-1721 monoclonal antibody recognizes the phosphorylated S383 of activated Elk-1. The orthologous phosphorylation sites of mouse and rat Elk-1 are S384 and S382, respectively.
Synonyms: ELK1

Pathways: MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling