CD40 antibody

Catalog No. ABIN1177310

Product Details anti-CD40 Antibody

Target: CD40

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD40 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Brand: BD Pharmingen™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Clone: 5C3

Isotype: IgG1 kappa

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-CD40 antibody (ABIN1177310)

Galy, Spits: "CD40 is functionally expressed on human thymic epithelial cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 3, pp. 775-82, (1992) (PubMed).

Itoh, Yonehara, Ishii, Yonehara, Mizushima, Sameshima, Hase, Seto, Nagata: "The polypeptide encoded by the cDNA for human cell surface antigen Fas can mediate apoptosis." in: Cell, Vol. 66, Issue 2, pp. 233-43, (1991) (PubMed).

Target Details for CD40

Target: CD40

Alternative Name: CD40 (CD40 Products)

Background: This 5C3 monoclonal antibody specifically binds to CD40, a 45-48 kDa type I integral membrane glycoprotein. CD40 is expressed on B lymphocytes, but is not expressed on terminally differentiated B cells. CD40 is also expressed by endothelial cells, basal epithelial cells and some epithelial cell carcinomas, follicular dendritic cells, macrophages, fibroblasts, keratinocytes, and CD34+ hematopoietic progenitor cells. This antibody is useful for studying the roles played by CD40 in B-cell growth, proliferation, and differentiation including immunoglobulin isotype switching. Anti-CD40 antibodies have been reported to stimulate B-cell proliferation when costimulated with anti-µ, anti-CD20 antibodies or with phorbol esters. 5C3 is capable of inducing B-cell proliferation when presented with IL-4.
Synonyms: TNFRSF5, TNF receptor superfamily member 5, CD40L receptor, Bp50, p50

Pathways: NF-kappaB Signaling, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints