RAD52 Homolog (S. Cerevisiae) (RAD52) (AA 360-375) antibody

Details for Product No. ABIN117934
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Antigen
Synonyms RAD52, zgc:112400, LOC100223085, MGC115453, Rad52yh
Epitope
AA 360-375
(11), (8), (8), (6), (6), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(70), (23), (12), (12), (12), (12), (12), (1)
Host
Rabbit
(65), (5)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
(59), (32), (17), (15), (13), (10), (4), (3), (2), (2), (1)
Pubmed 4 references available
Quantity 0.1 mg
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Catalog No. ABIN117934
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Immunogen This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to aa 360-375 of the Human Rad 52 protein.
Sequence Human RAD52, 419 aa, predicted MW 46.3 kDa: 1 msgteeailg grdshpaagg gsvlcfgqcq ytaeeyqaiq kalrqrlgpe yissrmaggg 61 qkvcyieghr vinlanemfg yngwahsitq qnvdfvdlnn gkfyvgvcaf vrvqlkdgsy 121 hedvgygvse glkskalsle karkeavtdg lkralrsfgn algncildkd ylrslnklpr 181 qlplevdltk akrqdlepsv eearynscrp nmalghpqlq qvtspsrpsh avipadqdcs 241 srslsssave seathqrklr qkqlqqqfre rmekqqvrvs tpsaekseaa ppappvthst 301 pvtvseplle kdflagvtqe liktlednse kwavtpdagd gvvkpssrad paqtsdtlal 361 nnqmvtqnrt phsvchqkpq aksgswdlqt ysadqrttgn weshrksqdm kkrkydpsy
Specificity This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. Reactivity occurs against human RAD52 protein. Cross reactivity with RAD52 protein from non-human sources is not expected based on a lack of homology (BLAST) with other known proteins.
Purification Immunoaffinity chromatography.
Alternative Name RAD52
Background The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNA recombination and repair. Four alternatively spliced transcript variants encoding different isoforms have been reported for this gene. The alpha variant encodes the longest isoform that contains an identical N-terminus, but a distinct C-terminus, as compared to other isoforms (beta, delta, and gamma).Synonyms: DNA repair protein RAD52 homolog
Gene ID 5893
NCBI Accession NP_602296
UniProt P43351
Research Area Chromatin and Nuclear Signaling, DNA/RNA
Application Notes This affinity purified antibody has been tested for use in Western blotting (1: 500-1: 2,000)using HeLa nuclear extracts. The antibody is also functional by ELISA (1: 5,000-1: 20,000). Dilutions for Western blotting represent a starting point dilution and further optimizationmay be required. The antibody detects a band of approximately 63.0 kDa (predictedmolecular weight: 46.3 kDa). Specific band detection by Western blot is blocked bypeptide competition by pre-incubating the antibody with the immunizing peptide prior toreaction with the membrane. Reactivity in other immunoassays is unknown.
Restrictions For Research Use only
Concentration 1.0 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8 °C as an undiluted liquid.
Supplier Images
anti-RAD52 Homolog (S. Cerevisiae) (RAD52) (AA 360-375) antibody Western blot analysis is shown using anti-Human RAD52 antibody to detect Human RAD52 present in a HeLa nuclear extract (panel B). ~30ug of lysate was loaded per lane for 4-20% gradient SDS-PAGE. Comparison to a molecular weight marker (not shown) indicates a band of ~63.0 kDa is detected. Peptide competition (panel A) blocks the specific staining of this band. The blot was incubated with a 1:1000 dilution of the antibody at room temperature for 2 h followed by detection using IRDye™800 labeled Goat-a-Rabbit IgG [H&L] diluted 1:5,000 for 45 min. IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
Background publications Muris, Bezzubova, Buerstedde et al.: "Cloning of human and mouse genes homologous to RAD52, a yeast gene involved in DNA repair and recombination." in: Mutation research, Vol. 315, Issue 3, pp. 295-305, 1994 (PubMed).

Shen, Denison, Lobb et al.: "The human and mouse homologs of the yeast RAD52 gene: cDNA cloning, sequence analysis, assignment to human chromosome 12p12.2-p13, and mRNA expression in mouse tissues." in: Genomics, Vol. 25, Issue 1, pp. 199-206, 1995 (PubMed).

Park: "Expression of human RAD52 confers resistance to ionizing radiation in mammalian cells." in: The Journal of biological chemistry, Vol. 270, Issue 26, pp. 15467-70, 1995 (PubMed).

General Zou, Yang, Chu et al.: "[Distribution of CSF1PO, TPOX and TH01 loci in Han Chinese]" in: Yi chuan xue bao = Acta genetica Sinica, Vol. 25, Issue 3, pp. 199-204, 1998 (PubMed).

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