DNA Cross-Link Repair 1C (DCLRE1C) (AA 482-495) antibody

Details for Product No. ABIN117935
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Antigen
Synonyms artemis, A-SCID, DCLREC1C, RS-SCID, SCIDA, SNM1C, hSNM1C, Snm1l, nuclease, 9930121L06Rik, AI661365, Art
Epitope
AA 482-495
(10), (9), (7), (5), (4), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(54), (16), (8), (1), (1)
Host
Goat
(49), (5)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(45), (32), (27), (13), (11), (3), (2), (1)
Pubmed 7 references available
Quantity 0.1 mg
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Catalog No. ABIN117935
390.50 $
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Immunogen This antibody was prepared from whole Goat serum produced by repeated immunizations with a synthetic peptide corresponding aa 482-495 of Human ARTEMIS (DCLRE1C DNA cross- link repair 1C). Genename: DCLRE1C
Specificity This antibody detects a band of the appropriate size in a number of different cell lines. However, it does not detect a band in cell lines that do not express ARTEMIS, such as the cell line CJ and in an hTERT line.
Cross-Reactivity (Details) Species reactivity (tested):Human.
Purification Immunoaffinity Chromatography using the immunizing peptide after immobilization to a solid phase.
Alternative Name Artemis
Background Artemis has a role in T and B lymphocyte immunodeficiency and in predisposition to lymphoma through the NHEJ pathway of DNA repair. DNAPKcs regulates Artemis by both phosphorylation and complex formation to permit enzymatic activities that are critical for the hairpin-opening step of V(D)J recombination and for the 5' and 3' overhang processing in non-homologous DNA end joining.Synonyms: A-SCID protein, ASCID, DCLRE1C, DNA cross-link repair 1C protein, SCIDA, SNM1-like protein, SNM1C
Gene ID 64421
NCBI Accession NP_001029027
UniProt Q96SD1
Research Area Chromatin and Nuclear Signaling, DNA/RNA
Application Notes This antibody has been tested for use in ELISA (1: 10,000-1: 20,000), Immunohistochemistry(1: 1,000) and Western blotting (1: 500-1: 2,000). Reactivity in other immunoassays isunknown. This antibody detects a band of approximately 90 kDa (predicted molecular weight: 78 kDa).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.2 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, with 0.01 %(w/v) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8 °C as an undiluted liquid.
Supplier Images
anti-DNA Cross-Link Repair 1C (DCLRE1C) (AA 482-495) antibody Immunohistochemical staining of ARTEMIS in Formalin fixed and Paraffin embeddes human spleen using antibody ABIN117935. Positive staining of T cells and B lymphocytes is observed in thymus, lymph nodes and spleen.
anti-DNA Cross-Link Repair 1C (DCLRE1C) (AA 482-495) antibody (2) Western blotting with anti-ARTEMIS antibody ABIN117935 at a 1:500 dilution in various cell lysates. CJ (lane 5) is an ARTEMIS deficient cell line and so no band is visible.
Background publications Moshous, Callebaut, de Chasseval et al.: "Artemis, a novel DNA double-strand break repair/V(D)J recombination protein, is mutated in human severe combined immune deficiency." in: Cell, Vol. 105, Issue 2, pp. 177-86, 2001 (PubMed).

Noordzij, Verkaik, van der Burg et al.: "Radiosensitive SCID patients with Artemis gene mutations show a complete B-cell differentiation arrest at the pre-B-cell receptor checkpoint in bone marrow." in: Blood, Vol. 101, Issue 4, pp. 1446-52, 2003 (PubMed).

Moshous, Pannetier, Chasseval Rd et al.: "Partial T and B lymphocyte immunodeficiency and predisposition to lymphoma in patients with hypomorphic mutations in Artemis." in: The Journal of clinical investigation, Vol. 111, Issue 3, pp. 381-7, 2003 (PubMed).

Kobayashi, Agematsu, Sugita et al.: "Novel Artemis gene mutations of radiosensitive severe combined immunodeficiency in Japanese families." in: Human genetics, Vol. 112, Issue 4, pp. 348-52, 2003 (PubMed).

Rooney, Alt, Lombard et al.: "Defective DNA repair and increased genomic instability in Artemis-deficient murine cells." in: The Journal of experimental medicine, Vol. 197, Issue 5, pp. 553-65, 2003 (PubMed).

Pannicke, Ma, Hopfner et al.: "Functional and biochemical dissection of the structure-specific nuclease ARTEMIS." in: The EMBO journal, Vol. 23, Issue 9, pp. 1987-97, 2004 (PubMed).

General Enders, Zieger, Schwarz et al.: "Lethal hemophagocytic lymphohistiocytosis in Hermansky-Pudlak syndrome type II." in: Blood, Vol. 108, Issue 1, pp. 81-7, 2006 (PubMed).

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