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Cullin 5 (CUL5) (N-Term), (AA 4-18) antibody

Details for Product No. ABIN117951
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cul5, wu:fd17d09, wu:fi20h12, xx:11fd17d09, zgc:66185, Xcullin5, CG1401, CUL5, Cul5, Cullin 5, Cullin5, Dmel\\CG1401, cul-5, vacm-1, vacm1, VACM-1, VACM1, 4921514I20Rik, 8430423K24Rik, AI852817, C0300 ... show more
cul5, wu:fd17d09, wu:fi20h12, xx:11fd17d09, zgc:66185, Xcullin5, CG1401, CUL5, Cul5, Cullin 5, Cullin5, Dmel\\CG1401, cul-5, vacm-1, vacm1, VACM-1, VACM1, 4921514I20Rik, 8430423K24Rik, AI852817, C030032G03Rik, C330021I08Rik, Cullin-5 show less
N-Term, AA 4-18
(7), (6), (3), (2), (2), (2), (1), (1), (1)
Human, Mouse (Murine)
(48), (30), (28), (24), (23), (11), (1), (1), (1), (1)
(48), (1)
(2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
ELISA, Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(26), (19), (13), (12), (7), (7), (3), (3), (2)
Pubmed 9 references available
Quantity 0.1 mL
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Catalog No. ABIN117951
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Immunogen Synthetic peptide corresponding to amino acids 4-18 of Human Cul5 (N-terminus) coupled coupled to KLH.
Sequence Human Cullin 5 (780 aa 90.9 kDa): 1 matsnllknk gslqfedkwd fmrpivlkll rqesvtkqqw fdlfsdvhav clwddkgpak 61 ihqalkedil efikqaqarv lshqddtall kayivewrkf ftqcdilpkp fcqleitlmg 121 kqgsnkksnv edsivrklml dtwnesifsn iknrlqdsam klvhaerlge afdsqlvigv 181 resyvnlcsn pedklqiyrd nfekayldst erfyrtqaps ylqqngvqny mkyadaklke 241 eekralryle trrecnsvea lmeccvnalv tsfketilae cqgmikrnet eklhlmfslm 301 dkvpngiepm lkdleehiis agladmvaaa etittdseky veqlltlfnr fsklvkeafq 361 ddprfltard kaykavvnda tifklelplk qkgvglktqp eskcpellan ycdmllrktp 421 lskkltseei eaklkevllv lkyvqnkdvf mryhkahltr rlildisads eieenmvewl 481 revgmpadyv nklarmfqdi kvsedlnqaf kemhknnkla lpadsvniki lnagawsrss 541 ekvfvslpte ledlipevee fykknhsgrk lhwhhlmsng iitfknevgq ydlevttfql 601 avlfawnqrp rekisfenlk latelpdael rrtlwslvaf pklkrqvlly epqvnspkdf 661 tegtlfsvnq efsliknakv qkrgkinlig rlqlttermr eeenegivql rilrtqeaii 721 qimkmrkkis naqlqtelve ilknmflpqk kmikeqiewl iehkyirrde sdintfiyma
Specificity This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human Cullin 5. Cross reactivity is expected against mouse Cul5 based on a high degree of sequence homology. Cross reactivity with other human cullins may occur.
Purification Delipidation and defibrination.
Alternative Name Cullin-5
Background Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates. Cullin 5 is a component of E3 ubiquitin ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. Cullin 5 seems to be involved proteosomal degradation of p53/TP53 stimulated by adenovirus E1B-55 kDa protein. Cullin 5 may form a cell surface vasopressin receptor. Cullin 5 is part of a E3 ubiquitin ligase complex with elongin BC complex (TCEB1 and TCEB2), RBX1 and MUF1, complexes with elongin BC complex (TCEB1 and TCEB2), RXB1 and TCEB3 or SOCS1 or WSB1, elongin BC complex (TCEB1 and TCEB2), RBX1 and VHL, elongin BC complex (TCEB1 and TCEB2), RBX1, adenovirus type 5 E1B-55kDa protein and adenovirus type 5 E4-orf6. Cullin 5 Interacts with RBX1 and RNF7.
Alternate names: CUL-5, CUL5, VACM-1, VACM1, Vasopressin-activated calcium-mobilizing receptor
Gene ID 8065
NCBI Accession NP_003469.2
UniProt Q93034
Application Notes Western blot (1/500-1/1,000): This antibody reacts with human Cul5. An 90.9 kDa bandcorresponding to human Cul5 is detected, Most cell lines expressing Cul5 can be used as a
Restrictions For Research Use only
Format Liquid
Concentration 85.0 mg/mL (by Refractometry)
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8°C for up to one month or in aliquots at -20°C for longer.
Expiry Date 12 months
Supplier Images
anti-Cullin 5 (CUL5) (N-Term), (AA 4-18) antibody Conjugation pathways for ubiquitin and ubiquitin-like modifiers (UBLs). Most modifiers mature by proteolytic processing from inactive precursors (a, amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins by RUB involves SCF(SKP1/cullin-1/F-box protein) /CBC(cullin-2/elongin B/elonginC) -like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin. Data contributed by S.Jentsch, see references below.
Background publications Kipreos, Lander, Wing et al.: "cul-1 is required for cell cycle exit in C. elegans and identifies a novel gene family." in: Cell, Vol. 85, Issue 6, pp. 829-39, 1996 (PubMed).

Mathias, Johnson, Winey et al.: "Cdc53p acts in concert with Cdc4p and Cdc34p to control the G1-to-S-phase transition and identifies a conserved family of proteins." in: Molecular and cellular biology, Vol. 16, Issue 12, pp. 6634-43, 1997 (PubMed).

Michel, Xiong: "Human CUL-1, but not other cullin family members, selectively interacts with SKP1 to form a complex with SKP2 and cyclin A." in: Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research, Vol. 9, Issue 6, pp. 435-49, 1998 (PubMed).

Ohta, Michel, Schottelius et al.: "ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity." in: Molecular cell, Vol. 3, Issue 4, pp. 535-41, 1999 (PubMed).

Jentsch, Pyrowolakis: "Ubiquitin and its kin: how close are the family ties?" in: Trends in cell biology, Vol. 10, Issue 8, pp. 335-42, 2000 (PubMed).

Querido, Blanchette, Yan et al.: "Degradation of p53 by adenovirus E4orf6 and E1B55K proteins occurs via a novel mechanism involving a Cullin-containing complex." in: Genes & development, Vol. 15, Issue 23, pp. 3104-17, 2001 (PubMed).

Liu, Furukawa, Matsumoto et al.: "NEDD8 modification of CUL1 dissociates p120(CAND1), an inhibitor of CUL1-SKP1 binding and SCF ligases." in: Molecular cell, Vol. 10, Issue 6, pp. 1511-8, 2002 (PubMed).

Yu, Yu, Liu et al.: "Induction of APOBEC3G ubiquitination and degradation by an HIV-1 Vif-Cul5-SCF complex." in: Science (New York, N.Y.), Vol. 302, Issue 5647, pp. 1056-60, 2003 (PubMed).

General Ng, Yu, Lai et al.: "Biliary Complications Associated with Selective Internal Radiation (SIR) Therapy for Unresectable Liver Malignancies." in: Digestive diseases and sciences, 2008 (PubMed).

Validation Images
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