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Details for Product No. ABIN118007

Caspase 2, Apoptosis-Related Cysteine Peptidase (CASP2) (N-Term) antibody

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Antigen
Synonyms CASP-2, ICH1, NEDD-2, NEDD2, PPP1R57, ICH-1, Nedd2, Caspase-2, ICH-1L/1S, ICH1L1S, xCaspase-2
Epitope
»Alternatives N-Term
Reactivity
»Alternatives Mouse (Murine), Rat (Rattus)
Host
»Alternatives Rabbit
Clonality Polyclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives ELISA, Western Blotting (WB)
Pubmed 4 references available
Catalog no. ABIN118007
Quantity 0.1 mg
Price
379.50 $   Plus shipping costs $45.00
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Availability Will be delivered in 6 to 8 Business Days
Immunogen A synthetic peptide corresponding to the N-terminus of rat Caspase-2 protein conjugated to Keyhole Limpet Hemocyanin (KLH) through a cysteine residue linker.
Sequence M-A-A-S-S-G-R-S-Q-S-S-L-H-R-K-G-L-C-KLH
Specificity This affinity purified antibody is directed against rat and mouse caspase-2. The antibody detects long and short forms of the protein. Antiserum was purified against the immunizing peptide. This antibody reacts with mouse and rat caspase-2, but not caspase-2 from human sources. Cross reactivity with caspase-2 from other sources has not been determined. Rat and mouse caspase-2 show 94% homology. There is no significant homology to the human form.
Purification Immunoaffinity chromatography.
Alternative Name Caspase-2
Background Apoptosis occurs during normal cellular development and involves dramatic changes in cellular structure. Disruption of apoptosis may contribute to cancer and other diseases. Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. Caspase-2 has been called ICH-1 in human, CED-3 in C.elegans and NEDD-2 in mouse. Caspase-2 is synthesized as an inactive precursor that is processed in cells undergoing apoptosis. The precursor form of all caspases is composed of a pro- domain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli, including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. Caspase-2 is activated in vitro by caspase-1, caspase-3 and granzyme B. Caspase-2 functions as an upstream apoptosis initiator that participates in the activation of downstream caspases, such as caspase-3, caspase-6 and caspase-7. Caspase-2 is highly expressed in embryonic mouse brain but not in adult neural tissue. Alternative mRNA splicing produces a long (48 kDa) and short (35 kDa) form of caspase-2. These forms show tissue specificity and function either as inducers or suppressors of apoptosis. Mature caspase-2 is 435 amino acids in length cleaved (~50 kDa) and is cleaved upon activation into 10 and 20 kDa subunits.
Alternate names: CASP-2, CASP2, ICH-1 protease, ICH1, NEDD2, Neural precursor cell expressed developmentally down-regulated protein 2
Gene ID 12366
NCBI Accession NP_031636.1
UniProt P29594
Research Area Apoptosis/Necrosis, Cancer, Autophagy
Application Notes This product was assayed against 1.0 g of immunizing peptide in a standard sandwichELISA using HRP conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and TMB as asubstrate for 30 minutes at room temperature. A working dilution of 1: 2,000 to 1: 10,000 issuggested for this product. This affinity purified polyclonal antibody was tested by immunoblotting and was found tobe reactive with 48 kDa mouse and rat caspase-2. A control and rat caspase-2 transfected human 293 cell lysate was used to show by
Restrictions For Research Use only
Format Liquid
Concentration 0.67 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% (w/v) Sodium Azide as preservative.
Preservative Sodium azide
Storage 4 °C
Storage Comment Store undiluted at 2-8°C.
Expiry Date 12 months
Background publications Wang, Miura, Bergeron et al.: "Ich-1, an Ice/ced-3-related gene, encodes both positive and negative regulators of programmed cell death." in: Cell, Vol. 78, Issue 5, pp. 739-50, 1994 (PubMed).

Li, Bergeron, Cryns et al.: "Activation of caspase-2 in apoptosis." in: The Journal of biological chemistry, Vol. 272, Issue 34, pp. 21010-7, 1997 (PubMed).

Cohen: "Caspases: the executioners of apoptosis." in: The Biochemical journal, Vol. 326 ( Pt 1), Issue 5693, pp. 1-16, 1997 (PubMed).

Bergeron, Perez, Macdonald et al.: "Defects in regulation of apoptosis in caspase-2-deficient mice." in: Genes & development, Vol. 12, Issue 9, pp. 1304-14, 1998 (PubMed).

Alternatives for antigen "Caspase 2, Apoptosis-Related Cysteine Peptidase (CASP2)", type "Antibodies"
Hosts (101), (31), (8)
Reactivities (135), (44), (31), (12), (12), (5), (2), (2), (1)
Applications (116), (59), (27), (21), (16), (14), (10), (9), (8), (3), (2), (1), (1), (1)
Conjugates (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (15), (10), (8), (6), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
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