CD161 antibody

Catalog No. ABIN118293

Product Details anti-CD161 Antibody

Target: CD161 (KLRB1) (Killer Cell Lectin-Like Receptor Subfamily B, Member 1 (KLRB1))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD161 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Functional Studies (Func)

Specificity: SM067A (Clone PK136) recognises the Mouse NK1.1 cell surface antigen also known as CD161. Although previously thought to recognise only CD161c, recent data has shown that the PK136 antibody may also react with CD161b. CD161c expression itself is strain specific in mice, but recognition of CD161b by PK136 appears to be even more complex, as only some CD161b positive strains are labelled by the antibody. Engagement of CD161c has been reported to have activating function in NK cells, whilst engagement of CD161b is inhibitory. PK136 is useful for the identification of NK cells in selected strains of mice (positive on C57BL, FVB/N and NZB, but negative on AKR and BALB/c) and is also expressed by rare subsets of T cells and monocytes. The antibody has also been used for in vivo depletion of NK cells and In Vitro activation of NK cells.

No Cross-Reactivity: Rat (Rattus), Human

Cross-Reactivity (Details): Species reactivity (tested):Mouse

Purification: Affinity Chromatography on Protein A

Immunogen: Spleen and bone marrow cells from CE mice

Clone: PK136

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry: use 10 μL of 5-10 μg/mL diluted antibody to label 1x10^6 cells in 100 μL. Complement dependent lysis 1-10 μg/mL (see Ref.4). In vivo depletion of NK cells (see Ref.4). Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, without preservatives

Preservative: Without preservative

Handling Advice: Dilute only prior to immediate use. Avoid cycles of freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD161

Target: CD161 (KLRB1) (Killer Cell Lectin-Like Receptor Subfamily B, Member 1 (KLRB1))

Alternative Name: CD161 / KLRB1 (KLRB1 Products)

Background: NK1.1 is a cell surface glycoprotein encoded by members of the NKR-P1 gene family. In the mouse the NKR-P1 family has three members, NKR-P1A, -B and -C, whilst in the human only one member has been identified. The human protein has received the designation CD161, and the mouse proteins have been referred to as CD161a, -b, -c etc.Synonyms: C-type lectin domain family 5 member B, CLEC5B, HNKR-P1a, Killer cell lectin-like receptor subfamily B member 1, NKRP1A, Natural killer cell surface protein P1A

Gene ID: 3820

NCBI Accession: NP_002249

UniProt: Q12918