CD3 antibody (PE)

Catalog No. ABIN118481

Product Details anti-CD3 Antibody (PE)

Target: CD3

Reactivity: Human, Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD3 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Specificity: This antibody recognises CD3, a 19 kDa cell surface protein which is expressed on more than 95 % of circulating peripheral blood T-cells.

Purification: Affinity chromatography on Protein G

Immunogen: Human infant thymocytes and lymphocytes from a patient with Sezary Syndrome.

Clone: UCHT1

Isotype: IgG1

Application Details

Application Notes: Flow cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with distilled water.

Concentration: 0.1 mg/mL

Buffer: PBS, pH 7.2 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: This product is photosensitive and should be protected from light

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!

Target Details for CD3

Target: CD3

Alternative Name: CD3 (CD3 Products)

Background: T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82 % of normal peripheral blood lymphocytes, 65-85 % of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.Synonyms: T-cell surface antigen T3/Leu-4, T-cell surface glycoprotein CD3, T3/Leu-4

Gene ID: 916

NCBI Accession: NP_000724

UniProt: P07766

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway