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F-Actin antibody

Details for Product No. ABIN118960, Supplier: Log in to see
Human, Mouse (Murine), Rabbit, Rat (Rattus)
Clonality (Clone)
Monoclonal ()
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
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Immunogen Human monocytes and U937 cell line. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Clone NH3
Isotype IgM
Specificity This antibody recognizes Human Filamentous Actin (F-actin). The antibody binds to the N-terminal region of Actin, but not to the extreme N-terminal 40 amino acids. In tissue sections the antibody stains the cytoplasm of macrophages strongly, and gives granular, localised nuclear staining of all cell types. Clone NH3 is reported to recognise Actin in the filamentous form with the epitope likely to be located between residues 120 and 226 of the molecule. Clone NH3 is also described to show reactivity with a 43 kDa polypeptide using cell lines U937 and HL-60 by SDS/PAGE and Immunoblotting.
Purification Purified
Alternative Name Actin (F-Actin)
Background Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. G-Actin (globular actin) with bound ATP can polymerise to form F-Actin (Filamentious Actin). Actin monomers spiral ariound the axis of the filament similar to a double helix. F-Actin may also undergo a process called treadmilling, in which filament length remains constant and actin monomers add at one end and dissociated at the other. Synonyms: Actin F type
Research Area Extracellular Matrix
Application Notes Flow Cytometry (1:10). Immunohistochemistry on frozen and paraffin sections. This product may require protein digestion pretreatment (e.g. trypsin or pronase) or microwave antigen retrieval prior to staining of paraffin sections. ELISA (1:10) Western blotting (1:100 - 1:500)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS, 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Background publications "Retraction." in: Endocrinology, 2010 (PubMed).

McCarthy, Spisak, Brozinick et al.: "Loss of cortical actin filaments in insulin-resistant skeletal muscle cells impairs GLUT4 vesicle trafficking and glucose transport." in: American journal of physiology. Cell physiology, Vol. 291, Issue 5, pp. C860-8, 2006 (PubMed).

Dransfield, Bradford, Smith et al.: "Ezrin is a cyclic AMP-dependent protein kinase anchoring protein." in: The EMBO journal, Vol. 16, Issue 1, pp. 35-43, 1997 (PubMed).