F-Actin antibody (N-Term)

Catalog No. ABIN2740760

Product Details anti-F-Actin Antibody

Target: F-Actin

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Rabbit

Host: Mouse

Clonality: Monoclonal

Conjugate: This F-Actin antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Recognizes human Filamentous Actin. Binds to the N-terminal region of actin, but not to the extreme N-terminal 40 amino acids. In tissue sections the antibody stains the cytoplasm of macrophages strongly and gives granular, localized nuclear staining of all cell types. Species cross-reactivity: Rabbit.

Purification: Purified

Immunogen: Human monocytes and U937 cell line.

Type of Immunogen: Cells

Isotype: IgM

Application Details

Application Notes: Approved: ELISA (1:5), Flo (1:5), IHC, IHC-Fr, IHC-P (15 μg/mL), WB

Usage: Suitable for use in Flow Cytometry, Immunohistochemistry, ELISA and Western Blot. Flow Cytometry: 1:5. Immunohistochemistry: frozen sections. ELISA: 1:5. Positive control: Gastrointestinal tissue.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for F-Actin

Target: F-Actin

Alternative Name: F Actin (F-Actin Products)