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KRT14 antibody (C-Term)

KRT14 Reactivity: Human WB, IF, FACS, IHC (p), IHC (fro) Host: Mouse Monoclonal LL002 unconjugated
Catalog No. ABIN118965
  • Target See all KRT14 Antibodies
    KRT14 (Keratin 14 (KRT14))
    Binding Specificity
    • 24
    • 15
    • 8
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivity
    • 93
    • 60
    • 46
    • 5
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 66
    • 31
    • 1
    Mouse
    Clonality
    • 54
    • 44
    Monoclonal
    Conjugate
    • 56
    • 6
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This KRT14 antibody is un-conjugated
    Application
    • 38
    • 28
    • 25
    • 24
    • 21
    • 16
    • 13
    • 13
    • 12
    • 10
    • 8
    • 5
    • 2
    • 2
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Specificity
    This antibody recognises Cytokeratin 14, a type I intermediate filament, expressed by stratifying epithelial cells and can be used to distinguish these cell types from simple epithelial cells, which do not express Cytokeratin 14.
    Purification
    Ion Exchange Chromatography
    Immunogen
    Last 15 C-terminal residues of Human Cytokeratin 14 conjugated to Thyroglobulin.
    Clone
    LL002
    Isotype
    IgG3
    Top Product
    Discover our top product KRT14 Primary Antibody
  • Application Notes
    Flow Cytometry: Use 10 μL of 1/100 diluted antibody to label 10^6 cells in 100 μL (Membrane permeabilisation is required). Western Blot. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections (1/200): This product requires antigen retrieval using heat treatment prior to staining of paraffin sections, Sodium citrate buffer is recommended for this purpose, Enzyme digestion is not recommended. Recommended Positive Control: Skin.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL
    Buffer
    PBS, 0.09 % Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Target
    KRT14 (Keratin 14 (KRT14))
    Alternative Name
    Cytokeratin 14 (KRT14 Products)
    Synonyms
    CK14 antibody, EBS3 antibody, EBS4 antibody, K14 antibody, NFJ antibody, AI626930 antibody, Krt-1.14 antibody, Krt1-14 antibody, Ka14 antibody, ouro2 antibody, keratin 14 antibody, keratin 14, type I L homeolog antibody, KRT14 antibody, Krt14 antibody, krt14.L antibody
    Background
    Cytokeratin is a type I intermediate filament, expressed by stratifying epithelial cells and can be used to distinguish these cell types from simple epithelial cells, which do not express cytokeratin 14. Keratin 14 is a member of the keratin family, the most diverse group of intermediate filaments. It is usually found as a heterotetramer with two molecules of keratin 5, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. Keratin 14 has been studied as a prognostic marker in breast cancer.Synonyms: CK14, Cytokeratin-14, K14, KRT14, Keratin 14, Keratin Type I Cytoskeletal 14
    Gene ID
    3861
    NCBI Accession
    NP_000517
    UniProt
    P02533
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