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Interleukin 4 (IL4) antibody

Details for Product No. ABIN119373
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Antigen
Synonyms BCGF-1, BCGF1, BSF-1, BSF1, IL-4, Il-4, Il4e12, IL2, IL4
Reactivity
Human
(204), (126), (36), (15), (8), (6), (4), (4), (3), (3), (2), (2), (2), (2), (2)
Host
Rat
(149), (116), (113), (4), (4), (3)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(50), (20), (11), (10), (6), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA, Western Blotting (WB), Flow Cytometry (FACS)
(251), (210), (100), (55), (45), (40), (34), (32), (23), (21), (20), (20), (18), (8), (7), (6), (6), (4), (4), (3), (3), (2), (1), (1), (1)
Pubmed 6 references available
Quantity 0.5 mg
Shipping to United States (Change)
Availability Will be delivered in 6 to 8 Business Days
Catalog No. ABIN119373
511.50 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Purified CHO cell expressed recombinant human IL-4.
Clone MP4-25D2
Isotype IgG1
Specificity This antibody is specific for Interleukin - 4 (IL-4). Clone MP4-25D2 is reported to neutralise IL-4 activity. We recommend the use of SM1702A for this purpose.
Purification Affinity chromatography on Protein G
Alternative Name Interleukin-4 / IL4
Background IL-4 is a 20kD cytokine which is expressed by a subpopulation of activated T cells and mast cells.
Alternate names: B-cell stimulatory factor 1, BSF1, Binetrakin, IL-4, Lymphocyte stimulatory factor 1, Pitrakinra
Gene ID 3565
NCBI Accession NP_000580.1
UniProt P05112
Application Notes Flow Cytometry: 1/10 - 1/50, Use 10ul of the suggested working dilution to label 10e6 cells in 100ul, Membrane permeabilisation is required for this application. ELISA. Western Blot: 1/500 - 1/1000. Immunohistochemistry on frozen sections. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS, pH7.4 containing 0.09% Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Supplier Images
anti-Interleukin 4 (IL4) antibody Western blot analysis of human IL-4 recombinant protein probed with RAT ANTI HUMAN INTERLEUKIN-4 (ABIN119373) followed by F(ab')2 RABBIT ANTI RAT IgG:HRP (SM1694A).
Background publications Krohn, Preis, Prehn: "Staurosporine-induced apoptosis of cultured rat hippocampal neurons involves caspase-1-like proteases as upstream initiators and increased production of superoxide as a main downstream effector." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 18, Issue 20, pp. 8186-97, 1998 (PubMed).

Bie, Yuan, Zhao et al.: "Method for estimating the axial intensity derivative in the TIE with higher order intensity derivatives and noise suppression." in: Optics express, Vol. 20, Issue 7, pp. 8186-91, 2012 (PubMed).

General Abrams, Roncarolo, Yssel et al.: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, 1992 (PubMed).

Carvalho, Bacellar, Brownell et al.: "Restoration of IFN-gamma production and lymphocyte proliferation in visceral leishmaniasis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 12, pp. 5949-56, 1994 (PubMed).

Kubota, Miller, Imaoka et al.: "Oral immunization with simian immunodeficiency virus p55gag and cholera toxin elicits both mucosal IgA and systemic IgG immune responses in nonhuman primates." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 11, pp. 5321-9, 1997 (PubMed).

Patterson, Behbahani, Kabat et al.: "Leukemia inhibitory factor inhibits HIV-1 replication and is upregulated in placentae from nontransmitting women." in: The Journal of clinical investigation, Vol. 107, Issue 3, pp. 287-94, 2001 (PubMed).

Hosts (149), (116), (113), (4), (4), (3)
Reactivities (204), (126), (36), (15), (8), (6), (4), (4), (3), (3), (2), (2), (2), (2), (2)
Applications (251), (210), (100), (55), (45), (40), (34), (32), (23), (21), (20), (20), (18), (8), (7), (6), (6), (4), (4), (3), (3), (2), (1), (1), (1)
Conjugates (50), (20), (11), (10), (6), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Epitopes (10), (5), (4), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN119373
511.50 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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