CD161 antibody

Catalog No. ABIN120521

Product Details anti-CD161 Antibody

Target: CD161 (KLRB1) (Killer Cell Lectin-Like Receptor Subfamily B, Member 1 (KLRB1))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD161 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Radioimmunoassay (RIA)

Cross-Reactivity (Details): Species reactivity (tested):Rat.

Purification: Purified

Immunogen: Purified splenic NK cells from the LEW rat strain.

Clone: 01-10-78

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: 1/50 - 1/100, use 10 μL of the suggested antibody solution to label 106 cells. Immunoprecipitation. Radioimmunoassay. Western blotting.
Other applications not tested.
Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, 0.09 % Sodium Azide

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2-8 °C for one month or (in small aliquots) at -20 °C for longer.

Target Details for CD161

Target: CD161 (KLRB1) (Killer Cell Lectin-Like Receptor Subfamily B, Member 1 (KLRB1))

Alternative Name: CD161 / KLRB1 (KLRB1 Products)

Background: Natural killer (NK) cells are lymphocytes that mediate cytotoxicity and secrete cytokines after immune stimulation. Several genes of the C-type lectin superfamily, including the rodent NKRP1 family of glycoproteins, are expressed by NK cells and may be involved in the regulation of NK cell function. The KLRB1 (CD161) protein contains an extracellular domain with several motifs characteristic of C type lectins, a transmembrane domain, and a cytoplasmic domain. The KLRB1 protein is classified as a type II membrane protein because it has an external C terminus. In mouse the NKRP1 family has three members, NKRP1A, B and C, whilst in human only one member has been identified. The human protein has received the designation CD161, and the mouse proteins have been referred to as CD161a, b and c. Engagement of CD161c has been reported to have activating function in NK cells, whilst engagement of CD161b is inhibitory.Synonyms: C-type lectin domain family 5 member B, CLEC5B, HNKR-P1a, Killer cell lectin-like receptor subfamily B member 1, NKRP1A, Natural killer cell surface protein P1A

Gene ID: 3820

NCBI Accession: NP_002249

UniProt: Q12918