IL-8 antibody

Catalog No. ABIN120891

Product Details anti-IL-8 Antibody

Target: IL-8 (IL8) (Interleukin 8 (IL8))

Reactivity: Sheep, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL-8 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Enzyme Immunoassay (EIA), Functional Studies (Func)

Specificity: This antibody recognises interleukin-8 and shows no cross-reactivity with ovine IL-1 beta, IL-6, MCP-1 or TNF alpha. This Interleukin 8 antibody neutralises the bioactivity of ovine IL-8.

Purification: Purified

Immunogen: Recombinant ovine IL-8

Clone: 8M6

Isotype: IgG2a

Application Details

Application Notes: ELISA: 5 μg/mL, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL, the antibody may be used in combination with ABIN182115 in ELISA assays for ovine IL-8. Western Blot. Flow Cytometry: 1/10, Removal of sodium azide is recommended prior to use in functional assays.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Expiry Date: 12 months

Target Details for IL-8

Target: IL-8 (IL8) (Interleukin 8 (IL8))

Alternative Name: Interleukin-8 / IL8 (IL8 Products)

Background: Interleukin 8, IL8 is a member of the CXC chemokine family. This family of small basic heparan-binding proteins are proinflammatory and primarily mediate the activation and migration of neutrophils into tissue from peripheral blood. This chemokine is one of the major mediators of the inflammatory response and is secreted by several cell types in response to an inflammatory stimulus. It functions as a chemoattractant, and is also a potent angiogenic factor. IL8 attracts neutrophils, basophils, and T-cells, but not monocytes. Cystic fibrosis (CF) is characterized by severe lung inflammation. The inflammatory process is believed to be caused by massive overproduction of the proinflammatory protein IL8, and the high levels of IL8 in the CF lung are therefore believed to be the central mechanism behind CF lung pathophysiology.Synonyms: CXCL8, Emoctakin, GCP1, Granulocyte chemotactic protein 1, MDNCF, MONAP, Monocyte-derived neutrophil chemotactic factor, Monocyte-derived neutrophil-activating peptide, NAP1, Neutrophil-activating protein 1, Protein 3-10C, T-cell chemotactic factor

Gene ID: 280828

Pathways: TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Hepatitis C, Autophagy