OLR1
Reactivity: Human
FACS
Host: Mouse
Monoclonal
15C4
APC
Application Notes
Immunohistochemistry on frozen sections. Immunoprecipitation. Flow Cytometry: 1:10, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Western Blot: 1:10 - 1/100, This antibody detects intact 50 kDa LOX-1 and also a prominent 30 kDa proteolytic fragment in western blotting. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.1 mg/mL
Buffer
PBS, 0.09 % Sodium Azide, 1 % BSA
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.
Expiry Date
12 months
Target
OLR1
(Oxidized Low Density Lipoprotein (Lectin-Like) Receptor 1 (OLR1))
LOX-1 is a 40-50 kDa class E scavenger receptor, expressed by endothelial cells, macrophages, activated platelets and smooth muscle cells. LOX-1 binds to a wide range of ligands, including oxidized low-density lipoprotein (oxLDL), hypochlorite modified high-density lipoprotein (HDL), aged/apoptotic cells, activated platelets and bacteria, reflecting its versatile physiological functions. Expression of the LOX-1 gene is upregulated by oxLDL and the binding of LOX-1 to oxLDL results in the activation of NF-kappaB. Furthermore, LOX-1 antibodies have been shown to suppress the oxidized HDL (oxHDL) activation of NF-KappaB in endothelial cells, suggesting that this activation may be due to the binding of oxHDL to LOX-1. Angiotensin II and the inflammatory cytokine Tumour Necrosis Factor alpha (TNFalpha) also invoke an increase in LOX-1 gene expression and studies have focused on its role in endothelial dysfunction (1,4) and inflammatory diseases such as atherosclerosis (1) and rheumatoid arthritis, as well as its affect on CD40/CD40L signaling in both atherosclerosis and human coronary artery endothelial cells (HCAECs).Synonyms: C-type lectin domain family 8 member A, CLEC8A, LOX-1, Lectin-like oxidized LDL receptor 1, Lectin-type oxidized LDL receptor 1, Ox-LDL receptor 1, Ox-LDL receptor 1 soluble form, Oxidized low-density lipoprotein receptor 1, sLOX-1, sLOX1