LRP1 antibody

Catalog No. ABIN125951

Product Details anti-LRP1 Antibody

Target: LRP1 (Low Density Lipoprotein Receptor-Related Protein 1 (LRP1))

Reactivity: Human, Mouse, Rat, Rabbit, Hamster

Host: Mouse

Clonality: Monoclonal

Conjugate: This LRP1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This monoclonal antibody 5A6 specifically reacts with the 85 kDa beta-chain of LRP/alpha2MR which is expressed in a restricted spectrum of cell types (for a recent review cf. Herz & Strickland). A strong immunohistochemical reaction is seen in hepatocytes, tissue macrophages, subsets of neurones and astrocytes in the central nervous system, fibroblasts, smooth muscle cells, and monocyte-derived foam cells in atherosclerotic lesions in the arterial wall. The antibody can also be used for the characterization of a subset of myelomonocytic subtypes of chronic and acute leukemia (CD 91). Cross-reacts with rabbit LDL receptor-related protein (LRP) or mouse LRP.

Purification: Affinity Chromatography on Protein A.

Immunogen: Human LRP/alpha2MR.

Clone: 5A6

Isotype: IgG2b

Application Details

Application Notes: Immunofluorescence. Immunohistochemistry on Frozen Sections (Dilute 1/20). Flow Cytometry. Immunoblotting. Neutralizing effects of 5A6 have also been reported (cf. Basu et al. , 2001).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in 1 mL dist. water

Buffer: Final solution contains 0.5 % BSA in PBS buffer, pH 7.4

Precaution of Use: Do Not Add Sodium Azide

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.

Target Details for LRP1

Target: LRP1 (Low Density Lipoprotein Receptor-Related Protein 1 (LRP1))

Alternative Name: CD91 / LRP1 (LRP1 Products)

Synonyms: A2MR antibody, APOER antibody, APR antibody, CD91 antibody, IGFBP3R antibody, LRP antibody, LRP1A antibody, TGFBR5 antibody, A2mr antibody, AI316852 antibody, Lrp antibody, LRP-1 antibody, LDL receptor related protein 1 antibody, prolow-density lipoprotein receptor-related protein 1 antibody, low density lipoprotein receptor-related protein 1 antibody, LRP1 antibody, Lrp1 antibody, LOC100414789 antibody

Background: Synonyms: A2MR, APOER, APR, Alpha-2-macroglobulin receptor, Apolipoprotein E receptor, Prolow-density lipoprotein receptor-related protein 1

Gene ID: 4035

NCBI Accession: NP_002323

UniProt: Q07954

Pathways: Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling