Low Density Lipoprotein Receptor-Related Protein 1 (LRP1) antibody

Details for Product No. ABIN125951
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Antigen
Synonyms A2MR, APOER, APR, CD91, IGFBP3R, LRP, LRP1A, TGFBR5, A2mr, AI316852, Lrp, LRP-1
Reactivity
Hamster, Human, Mouse (Murine), Rabbit, Rat (Rattus)
(131), (47), (31), (13), (12), (12), (5), (1), (1)
Host
Mouse
(71), (63)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(15), (11), (8), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB), Flow Cytometry (FACS)
(69), (56), (50), (34), (32), (30), (20), (17), (15), (10), (8), (2), (1), (1)
Pubmed 10 references available
Quantity 50 μg
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Catalog No. ABIN125951
456.50 $
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Immunogen Human LRP/alpha2MR.
Clone 5A6
Isotype IgG2b
Specificity This monoclonal antibody 5A6 specifically reacts with the 85 kD beta-chain of LRP/alpha2MR which is expressed in a restricted spectrum of cell types (for a recent review cf. Herz & Strickland). A strong immunohistochemical reaction is seen in hepatocytes, tissue macrophages, subsets of neurones and astrocytes in the central nervous system, fibroblasts, smooth muscle cells, and monocyte-derived foam cells in atherosclerotic lesions in the arterial wall. The antibody can also be used for the characterization of a subset of myelomonocytic subtypes of chronic and acute leukemia (CD 91). Cross-reacts with rabbit LDL receptor-related protein (LRP) or mouse LRP.
Purification Affinity Chromatography on Protein A.
Alternative Name CD91 / LRP1
Background CD91 is expressed in a restricted spectrum of cell types (for a recent review cf. Herz &Strickland). A strong immunohistochemical reaction is seen in hepatocytes, tissuemacrophages, subsets of neurones and astrocytes in the central nervous system,fibroblasts, smooth muscle cells, and monocyte-derived foam cells in atheroscleroticlesions in the arterial wall. The antibody can also be used for the characterization of asubset of myelomonocytic subtypes of chronic and acute leukemia (CD 91).
Alternate names: A2MR, APOER, APR, Alpha-2-macroglobulin receptor, Apolipoprotein E receptor, Prolow- density lipoprotein receptor-related protein 1
Gene ID 4035
NCBI Accession NP_002323
UniProt Q07954
Research Area CD Antigens, Surface Receptors of Immune Cells, Receptors
Application Notes Immunofluorescence. Immunohistochemistry on Frozen Sections (Dilute 1/20). Flow Cytometry. Immunoblotting. Neutralizing effects of 5A6 have also been reported (cf. Basu et al. , 2001). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Reconstitution Restore in 1 mL dist. water
Buffer Final solution contains 0.5% BSA in PBS buffer, pH 7.4
Precaution of Use Do Not Add Sodium Azide
Handling Advice Avoid repeated freezing and thawing. Dilute only prior to immediate use
Storage 4 °C/-20 °C
Storage Comment Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8°C as an undiluted liquid.
Expiry Date 12 months
Background publications Wolf, Lopes, VandenBerg et al.: "Characterization and immunohistochemical localization of alpha 2-macroglobulin receptor (low-density lipoprotein receptor-related protein) in human brain." in: The American journal of pathology, Vol. 141, Issue 1, pp. 37-42, 1992 (PubMed).

Strickland, Ashcom, Williams et al.: "Sequence identity between the alpha 2-macroglobulin receptor and low density lipoprotein receptor-related protein suggests that this molecule is a multifunctional receptor." in: The Journal of biological chemistry, Vol. 265, Issue 29, pp. 17401-4, 1990 (PubMed).

Coukos, Gåfvels, Wisel et al.: "Expression of alpha 2-macroglobulin receptor/low density lipoprotein receptor-related protein and the 39-kd receptor-associated protein in human trophoblasts." in: The American journal of pathology, Vol. 144, Issue 2, pp. 383-92, 1994 (PubMed).

Strickland, Kounnas, Argraves: "LDL receptor-related protein: a multiligand receptor for lipoprotein and proteinase catabolism." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 9, Issue 10, pp. 890-8, 1995 (PubMed).

Misra, Gawdi, Pizzo: "Ligation of low-density lipoprotein receptor-related protein with antibodies elevates intracellular calcium and inositol 1,4, 5-trisphosphate in macrophages." in: Archives of biochemistry and biophysics, Vol. 372, Issue 2, pp. 238-47, 2000 (PubMed).

Basu, Binder, Ramalingam et al.: "CD91 is a common receptor for heat shock proteins gp96, hsp90, hsp70, and calreticulin." in: Immunity, Vol. 14, Issue 3, pp. 303-13, 2001 (PubMed).

Berl, Huc, Khoury et al.: "Helical molecular programming: supramolecular double helices by dimerization of helical oligopyridine-dicarboxamide strands." in: Chemistry (Weinheim an der Bergstrasse, Germany), Vol. 7, Issue 13, pp. 2810-20, 2001 (PubMed).

Herz, Strickland: "LRP: a multifunctional scavenger and signaling receptor." in: The Journal of clinical investigation, Vol. 108, Issue 6, pp. 779-84, 2001 (PubMed).

Radsak, Hilf, Singh-Jasuja et al.: "The heat shock protein Gp96 binds to human neutrophils and monocytes and stimulates effector functions." in: Blood, Vol. 101, Issue 7, pp. 2810-5, 2003 (PubMed).

General Rebeck, Reiter, Strickland et al.: "Apolipoprotein E in sporadic Alzheimer's disease: allelic variation and receptor interactions." in: Neuron, Vol. 11, Issue 4, pp. 575-80, 1993 (PubMed).

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