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Details for Product No. ABIN125989

Keratin 18 (KRT18) antibody

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Antigen
Synonyms K18, CK18, Krt1-18, CYK18, cb83, dapk1, DreK18, sb:cb83, wu:fa13f02, wu:fb36b04, krt18, MGC64569, MGC75922, KRT18
Reactivity
»Alternatives Cow (Bovine), Human
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA, Western Blotting (WB)
Pubmed 3 references available
Catalog no. ABIN125989
Quantity 50 µg
Price
401.50 $   Plus shipping costs $45.00
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Availability Will be delivered in 6 to 8 Business Days
Immunogen Cytoskeletal preparation from tumor cell line MCF-7.
Clone Ks18-27
Isotype IgG1
Specificity Ks 18.27 represents an excellent marker to discriminate simple epithelia from those of different origin. Polypeptide reacting: Mr 45,000 polypeptide (keratin K18, formerly also designated cytokeratin 18) of all simple type and complex epithelia. Reacts with human, bovine, negative with mouse. Reacts with MCF-7, HeLa. A-431, PLC, RT 112. All adeno-CA tested, undifferentiated CA, cervix CA, hepatocellular CA.
Purification Protein A affinity chromatography
Alternative Name Cytokeratin 18
Background Cytokeratin 18 is an acidic keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. Cytokeratin 18 exists in combination with Cytokeratin 8, a basic keratin. Hepatocellular carcinomas have been reportedly defined by the use of antibodies that recognize only Cytokeratins 8 and 18.
Alternate names: CK18, CYK18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, K18, KRT18, Keratin 18, Keratin type I cytoskeletal 18, Keratin-18
Gene ID 3875
NCBI Accession NP_000215.1
UniProt P05783
Research Area Signaling, Cytoskeleton, Extracellular Matrix
Application Notes Immunohistochemistry on frozen sections: 1: 10, 1 h at RT. Cytological material. Immunoblotting. ELISA. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Restore in 1 mL dist. water
Buffer PBS buffer, pH 7.4 containing 0.09 % sodium azide, 0.5% BSA
Preservative Sodium azide
Handling Advice This product is photosensitive and should be protected from light
Storage 4 °C
Storage Comment Prior to and following reconstitution store the antibody undiluted at 2-8°C. DO NOT FREEZE!
Expiry Date 12 months
Background publications Moll, Schiller, Franke: "Identification of protein IT of the intestinal cytoskeleton as a novel type I cytokeratin with unusual properties and expression patterns." in: The Journal of cell biology, Vol. 111, Issue 2, pp. 567-80, 1990 (PubMed).

Moll, Achtstätter, Becht et al.: "Cytokeratins in normal and malignant transitional epithelium. Maintenance of expression of urothelial differentiation features in transitional cell carcinomas and bladder carcinoma cell culture lines." in: The American journal of pathology, Vol. 132, Issue 1, pp. 123-44, 1988 (PubMed).

Moll, Franke, Schiller et al.: "The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells." in: Cell, Vol. 31, Issue 1, pp. 11-24, 1983 (PubMed).

Alternatives for antigen "Keratin 18 (KRT18)", type "Antibodies"
Hosts (276), (188), (9), (4), (3)
Reactivities (421), (114), (102), (43), (42), (34), (28), (17), (12), (8), (6), (6), (4), (4), (3), (3), (3), (3), (2), (1)
Applications (320), (227), (151), (113), (110), (104), (77), (64), (62), (50), (34), (8), (3), (1), (1), (1), (1), (1)
Conjugates (39), (29), (6), (5), (5), (5), (5), (5), (5), (5), (5), (1), (1)
Epitopes (26), (18), (17), (12), (12), (9), (5), (5), (5), (4), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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