Synaptophysin (SYP) antibody

Antigen: Synaptophysin (SYP)

Synonyms: Syn, p38, AI848995, A230093K24Rik, Syp1, syp-A, xsypi, MGC86277, syp-B, xsypii, MGC86267

Clonality: Monoclonal (SY38)

Host: Mouse

Reactivity: Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)

Conjugate: Un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN126707

Additional Information

Alternative name: Synaptophysin / SYP

UniProt: P08247

Immunogen: Synaptophysin from presynaptic vesicles, prepared from bovine brain.

Cross-Reactivity: Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)

Isotype: IgG1

Clone: SY38

Description: Synaptophysin is a glycoprotein present in the membrane of neuronal presynaptic vesiclesin brain, spinal cord, retina, vesicles of adrenal medulla, neuromuscular junctions, andendocrine cells. It is also expressed by neuroendocrine cells throughout the body, bothnormal and neoplastic. Synaptophysin is a useful marker for the identification of normalneuroendocrine cells and neuroendocrine neoplasms. This antibody reacts withneuroendocrine cells of human adrenal medulla, carotid body, skin, pituitary gland,thyroid, lung, pancreas, and gastointestinal mucosa. It also reacts with a wide spectrum ofneuroendocrine neoplasms of neural type including neuroblastomas,ganglioneuroblastomas, ganglioneuromas, pheochromocytomas, chromaffin, and nonchromaffin paragangliomas.

Characteristics: Synonyms: Neuroendocrine Marker, Major synaptic vesicle protein p38

Application Details

Application Notes: Immunoblotting. Immunofluorescence. Cytological Material. Immunohistochemistry on Frozen and Paraffin Sections: 1/50 with PBS, pH 7.4. In no case Protease Pretreatment. Incubation Time: 1 h at RT, extended with Paraffin. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Purification: Purified

Buffer: Final Solution contains PBS, pH 7.4 with 0.09% Sodium Azide aspreservative and 0.5% BSA as stabilizer.Reconstitution: Restore in 1 ml distilled water.

Storage: Prior to reconstitution store at 2-8°C. Following reconstitution store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Restrictions: For Research Use only

Publications

Wiedenmann, Franke, Kuhn et al.: "Synaptophysin: a marker protein for neuroendocrine cells and neoplasms." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, Issue 10, pp. 3500-4, 1986 (PubMed).

Gould, Lee, Wiedenmann et al.: "Synaptophysin: a novel marker for neurons, certain neuroendocrine cells, and their neoplasms." in: Human pathology, Vol. 17, Issue 10, pp. 979-83, 1986 (PubMed).

Gould, Wiedenmann, Lee et al.: "Synaptophysin expression in neuroendocrine neoplasms as determined by immunocytochemistry." in: The American journal of pathology, Vol. 126, Issue 2, pp. 243-57, 1987 (PubMed).

Gould: "Synaptophysin. A new and promising pan-neuroendocrine marker." in: Archives of pathology & laboratory medicine, Vol. 111, Issue 9, pp. 791-4, 1987 (PubMed).

Kelly, Delclos, Morice et al.: "High-dose-rate endobronchial brachytherapy effectively palliates symptoms due to airway tumors: the 10-year M. D. Anderson cancer center experience." in: International journal of radiation oncology, biology, physics, Vol. 48, Issue 3, pp. 697-702, 2000 (PubMed).