Biotin antibody (PE)

Catalog No. ABIN129563

Supplier Product No.: 600-108-098

Product Details anti-Biotin Antibody (PE)

Target: Biotin

Reactivity: Please inquire

Host: Goat

Clonality: Polyclonal

Conjugate: This Biotin antibody is conjugated to PE

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Supplier Product No.: 600-108-098

Supplier: Rockland

Purpose: Biotin Antibody Phycoerythrin Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Phycoerythrin, Biotin conjugated IgG and Biotin conjugated Bovine Serum Albumin.

Characteristics: Synonyms: goat anti-Biotin Antibody PE Conjugation, Phycoerythrin conjugated goat anti-Biotin Antibody, Vitamin B4 antibody

Purification: Anti-Biotin Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads.

Immunogen:

Immunogen: Biotin conjugated to Keyhole Limpet Hemocyanin (b-KLH)

Immunogen Type: Other

Isotype: IgG

Application Details

Application Notes:

Flow Cytometry Dilution: 1.0 μg/1,000,000 cells

Immunohistochemistry Dilution: User Optimized

Application Note: Anti-Biotin detects biotin and biocytin by ELISA, Immuno-histo-chemistry (IHC), and the antibody also recognizes biotin conjugated to various immunoglobulins. The antibody may be used in amplification techniques, immunoblotting, in situ nucleic acid hybridization, flow cytometry or Immunofluorescence microscopy.

Western Blot Dilution: 1:50-1:200

ELISA Dilution: 1:50-1:200

IF Microscopy Dilution: 1:50-1:200

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 0.5 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.

Expiry Date: 6 months

Target Details for Biotin

Target: Biotin

Abstract: Biotin Products

Target Type: Chemical

Background: Background: Biotin Antibody detects Biotin. Biotin is a water-soluble B-complex vitamin (vitamin B7). It is composed of a ureido (tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring. Biotin is a coenzyme for carboxylase enzymes, involved in the synthesis of fatty acids, isoleucine, and valine, and in gluconeogenesis. Biotin is necessary for cell growth, the production of fatty acids, and the metabolism of fats and amino acids. Anti-Biotin Phycoerythrin Conjugated Antibody is ideal for investigators involved in Cell Signaling and Cell Biology research.