Red Fluorescent Protein (RFP) (AA 234) antibody

Details for Product No. ABIN129578
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Antigen
Synonyms RFP, RNF76
Epitope
AA 234
(5), (3), (3), (1)
Reactivity
Discosoma
(25), (9), (4), (1), (1)
Host
Rabbit
(19), (15), (5), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(4), (2), (2), (1)
Application
Western Blotting (WB), ELISA, Fluorescence Microscopy (FM)
(36), (28), (10), (4), (4), (4), (4), (3), (1)
Pubmed 8 references available
Quantity 100 µg
Options
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Catalog No. ABIN129578
240.90 $
Plus shipping costs $45.00 and if applicable $26.40 dry ice

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Immunogen The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence derived from the mushroom polyp coral Discosoma.
Immunogentype:Recombinant
Isotype IgG
Characteristics Concentration Definition: by UV absorbance at 280 nm
Purification Pre-adsorbed
Alternative Name RFP
Background Antibodies to RFP (Discosoma spp.) are intended for use in immunological assays including ELISA, western blotting, fluorometry and fluorescence activated cell sorting (FACS).
Synonyms: DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583
Research Area Tags/Labels
Application Notes Polyclonal anti-RFP is designed to detect RFP and its variants. This antibody can be used to detect RFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated polyclonal anti-RFP used in a sandwich ELISA with unconjugated anti-RFP is well suited to titrate RFP in solution. The detection antibody conjugated to biotin is subsequently reacted with streptavidin conjugated HRP Fluorochrome conjugated polyclonal anti-RFP can be used to detect RFP by immunofluorescence microscopy in cell expression systems and can detect RFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-RFP relative to the fluorescence of RFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-RFP to detect RFP or RFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-Red Fluorescent Protein (RFP) (AA 234) antibody Western blot of RFP recombinant protein detected with polyclonal anti-RFP antibody. Lane 1 shows no reaction against a GFP recombinant protein present in 10 ?g of HeLa cell extract. Lane 2 shows a single band detected in 10 ?g of a HeLa lysate containing RFP recombinant protein as a 27 kDa band. A 4-12% Bis-Tris gradient gel (Invitrogen) was used for SDS-PAGE. The membrane was blocked and then probed with Anti-RFP diluted 1:2,500 for 1 h at RT followed by washes and reaction with a 1:5,000 dilution of IRDye™800 conjugated Goat-a-Rabbit IgG [H&L] MX (611-132-122). IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
Product cited in: Yoshinaga, Ohkubo, Sasaki et al.: "A Phosphatidylinositol Lipids System, Lamellipodin, and Ena/VASP Regulate Dynamic Morphology of Multipolar Migrating Cells in the Developing Cerebral Cortex." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 32, Issue 34, pp. 11643-56, 2012 (PubMed).

Viganò, Möbius, Götz et al.: "Transplantation reveals regional differences in oligodendrocyte differentiation in the adult brain." in: Nature neuroscience, Vol. 16, Issue 10, pp. 1370-2, 2013 (PubMed). (1:500).

General Shi, Wu, Liu et al.: "Chitosan fibers: versatile platform for nickel-mediated protein assembly." in: Biomacromolecules, Vol. 9, Issue 5, pp. 1417-23, 2008 (PubMed).

Dallaire, Bellmann, Laplante et al.: "Obese mice lacking inducible nitric oxide synthase are sensitized to the metabolic actions of peroxisome proliferator-activated receptor-gamma agonism." in: Diabetes, Vol. 57, Issue 8, pp. 1999-2011, 2008 (PubMed).

Burton, Johnson, Sato et al.: "Adenovirus-mediated gene expression imaging to directly detect sentinel lymph node metastasis of prostate cancer." in: Nature medicine, Vol. 14, Issue 8, pp. 882-8, 2008 (PubMed).

Becher, Hambardzumyan, Walker et al.: "Preclinical evaluation of radiation and perifosine in a genetically and histologically accurate model of brainstem glioma." in: Cancer research, Vol. 70, Issue 6, pp. 2548-57, 2010 (PubMed).

Fries, Reddy, Mikhaylova et al.: "Dynamic cellular translocation of caldendrin is facilitated by the Ca2+-myristoyl switch of recoverin." in: Journal of neurochemistry, Vol. 113, Issue 5, pp. 1150-62, 2010 (PubMed).

Chambers, Takimoto: "Trafficking of Sendai virus nucleocapsids is mediated by intracellular vesicles." in: PLoS ONE, Vol. 5, Issue 6, pp. e10994, 2010 (PubMed).

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