RFP antibody (Red Fluorescent Protein) Primary Antibody
- This RFP antibody is un-conjugated
- ELISA, Western Blotting (WB)
- Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry.
- Anti-RFP Monoclonal Antibody was purified from concentrated tissue culture supernate by Protein A chromatography.
Anti-RFP monoclonal antibody is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.
Immunogen Type: Recombinant Protein
anti-Red Fluorescent Protein (RFP) antibody (Abberior STAR 635P) Primary Antibody
RFP Reactivity: Discosoma FACS, ICC, IF Host: Camelid (Camelidae) Monoclonal 2B12-2A1 Abberior STAR 635P single-domain Antibody (sdAb)
- Application Notes
Anti-RFP antibodies is designed to detect Red Fluorescent Protein and its variants. This antibody can be used to detect RFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated anti-RFP used in a sandwich ELISA with unconjugated anti-RFP is well suited to titrate RFP in solution. The detection antibody conjugated to biotin is subsequently reacted with streptavidin conjugated HRP. Fluorochrome conjugated anti-RFP can be used to detect RFP by immunofluorescence microscopy in cell expression systems and can detect RFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated anti-RFP relative to the fluorescence of RFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated anti-RFP to detect RFP or RFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher.
ELISA Dilution: 1:20.000-1:50.000
Western Blot Dilution: 1:1000-1:3000
- For Research Use only
- 1 mg/mL
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- Handling Advice
- Avoid cycles of freezing and thawing.
- 4 °C/-20 °C
- Storage Comment
- Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- Expiration date is one (1) year from date of opening.
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- Antibodies to RFP (Discosoma spp.) are intended for use in immunological assays including ELISA, western blotting, fluorometry and fluorescence activated cell sorting (FACS). Synonyms: DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583