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CD8A antibody (CD8 Antigen, alpha Chain)

Details for Product anti-CD8A Antibody No. ABIN135118, Supplier: Log in to see
Antigen
  • BB154331
  • Ly-2
  • Ly-35
  • Ly-B
  • Lyt-2
  • zgc:136643
  • cd8
  • LOC100136450
Reactivity
Mouse (Murine)
38
Host
Rat
38
Clonality (Clone)
Monoclonal ()
Conjugate
This CD8A antibody is un-conjugated
7
6
4
2
2
2
2
2
2
2
1
1
1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC)
37
14
2
2
Supplier
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Clone 53-6-7
Isotype IgG2a
Characteristics The antibody detects: chain of the murine CD8 heterodimer, Mr 32-34 kDa
The 53-6.7 monoclonal antibody also blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2.
Purification Purified
Alternative Name CD8a/Ly-2 (CD8A Antibody Abstract)
Background In the mouse, CD8 exists in two forms: (i) a CD8 heterodimer composed of an chain (CD8/Lyt-2) and a chain (CD8/Lyt-3), and (ii) a homodimer of two chains. The heterodimer is found on the surface of essentially all thymocytes and the suppressor/cytotoxic subpopulation of mature T lymphocytes. Subsets of intestinal intraepithelial lymphocytes express CD8 without CD8. It has been suggested that CD8+- T cells mature extrathymically, while development of the CD8++ population of T cells is thymus-dependent. CD8 acts as a coreceptor with MHC Class I-restricted T cell receptors in antigen recognition and positive selection of MHC class I-restricted CD8+ T cells. In vivo and in vitro treatment with the 53-6.7 monoclonal antibody effectively depletes CD8+ cells.
Pathways
Application Notes Working Dilution:
FACS: 2 µg/10^6 cells
Representative data are included in this product insert.

Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Comment

In vivo and in vitro depletion of CD8a+ cells

Sample Volume 1 mL
Restrictions For Research Use only
Buffer Product is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.0.
Preservative Azide free
Handling Advice Each reagent is stable for the period shown on the bottle label if stored as directed.
Storage 4 °C
Supplier Images
 image for anti-CD8A antibody (CD8 Antigen, alpha Chain) (ABIN135118) anti-CD8 Antigen, alpha Chain (CD8A) antibody
Product cited in: Jordan, Buhrman, Sprague et al.: "TCR hypervariable regions expressed by T cells that respond to effective tumor vaccines." in: Cancer immunology, immunotherapy : CII, 2012 (PubMed).

Background publications Ledbetter, Seaman: "The Lyt-2, Lyt-3 macromolecules: structural and functional studies." in: Immunological reviews, Vol. 68, pp. 197-218, 1983 (PubMed).

Ledbetter, Rouse, Micklem et al.: "T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views." in: The Journal of experimental medicine, Vol. 152, Issue 2, pp. 280-95, 1980 (PubMed).

Takahasi, Kondou, Watanabe et al.: "[Investigation of etching and bonding on the inner surface of free enamel. SEM observation and thermal cycling test for pigment invasion]" in: Shika gakuho. Dental science reports, Vol. 89, Issue 3, pp. 663-8, 1990 (PubMed).

Lefrançois: "Extrathymic differentiation of intraepithelial lymphocytes: generation of a separate and unequal T-cell repertoire?" in: Immunology today, Vol. 12, Issue 12, pp. 436-8, 1992 (PubMed).

Lefrancois: "Phenotypic complexity of intraepithelial lymphocytes of the small intestine." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 147, Issue 6, pp. 1746-51, 1991 (PubMed).