CD8 Antigen, alpha Chain (CD8A) antibody

Details for Product No. ABIN135118
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Antigen
Synonyms Ly-2, Ly-B, Ly-35, Lyt-2, BB154331, zgc:136643, cd8, LOC100136450
Reactivity
Mouse (Murine)
(33)
Host
Rat
(33)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(6), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application
Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP)
(32), (14), (2), (2)
Pubmed 6 references available
Catalog no. ABIN135118
Quantity 0.5 mg
Price
209.00 $   Plus shipping costs $45.00
Options
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  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Clone 53-6-7
Isotype IgG2a
Characteristics The antibody detects: chain of the murine CD8 heterodimer, Mr 32-34 kDa
The 53-6.7 monoclonal antibody also blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2.
Purification Purified
Purity Purified
Alternative Name CD8a/Ly-2
Background In the mouse, CD8 exists in two forms: (i) a CD8 heterodimer composed of an chain (CD8/Lyt-2) and a chain (CD8/Lyt-3), and (ii) a homodimer of two chains. The heterodimer is found on the surface of essentially all thymocytes and the suppressor/cytotoxic subpopulation of mature T lymphocytes. Subsets of intestinal intraepithelial lymphocytes express CD8 without CD8. It has been suggested that CD8+- T cells mature extrathymically, while development of the CD8++ population of T cells is thymus-dependent. CD8 acts as a coreceptor with MHC Class I-restricted T cell receptors in antigen recognition and positive selection of MHC class I-restricted CD8+ T cells. In vivo and in vitro treatment with the 53-6.7 monoclonal antibody effectively depletes CD8+ cells.
Application Notes Working Dilution:
FACS: 2 µg/10^6 cells
Representative data are included in this product insert.

Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Comment

In vivo and in vitro depletion of CD8a+ cells

Sample Volume 1 mL
Restrictions For Research Use only
Buffer Product is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.0.
Preservative Azide free
Handling Advice Each reagent is stable for the period shown on the bottle label if stored as directed.
Storage 4 °C
Supplier Images
anti-CD8 Antigen, alpha Chain (CD8A) antibody anti-CD8 Antigen, alpha Chain (CD8A) antibody
Product cited in: Jordan, Buhrman, Sprague et al.: "TCR hypervariable regions expressed by T cells that respond to effective tumor vaccines." in: Cancer immunology, immunotherapy : CII, 2012 (PubMed).

General Lefrancois: "Phenotypic complexity of intraepithelial lymphocytes of the small intestine." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 147, Issue 6, pp. 1746-51, 1991 (PubMed).

Lefrançois: "Extrathymic differentiation of intraepithelial lymphocytes: generation of a separate and unequal T-cell repertoire?" in: Immunology today, Vol. 12, Issue 12, pp. 436-8, 1992 (PubMed).

Takahasi, Kondou, Watanabe et al.: "[Investigation of etching and bonding on the inner surface of free enamel. SEM observation and thermal cycling test for pigment invasion]" in: Shika gakuho. Dental science reports, Vol. 89, Issue 3, pp. 663-8, 1990 (PubMed).

Ledbetter, Rouse, Micklem et al.: "T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views." in: The Journal of experimental medicine, Vol. 152, Issue 2, pp. 280-95, 1980 (PubMed).

Ledbetter, Seaman: "The Lyt-2, Lyt-3 macromolecules: structural and functional studies." in: Immunological reviews, Vol. 68, pp. 197-218, 1983 (PubMed).

Hosts (33)
Reactivities (33)
Applications (32), (14), (2), (2)
Conjugates (6), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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