CD28 antibody (Biotin)

Catalog No. ABIN135534

Product Details anti-CD28 Antibody (Biotin)

Target: CD28

Reactivity: Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD28 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Specificity: Chicken/Turkey CD28, Mr 40-44 kDa

Characteristics: Mouse Anti-Chicken CD28-BIOT

Immunogen: Chicken peripheral blood lymphocytes

Clone: AV7

Isotype: IgG1

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , ICC - Reported in literature , IP - Reported in literature , Costim - Reported in literature
• Working Dilutions: Flow Cytometry FITC and BIOT conjugates 1 g/106 cells PE conjugate 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Comment:

In vitro T-cell costimulation

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg in 1.0 mL of PBS/Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

Target Details for CD28

Target: CD28

Alternative Name: CD28 (CD28 Products)

Background: Chicken CD28 antigen is a type I transmembrane monomeric glycoprotein which does not contain the cysteine residues that form disulfide-linked covalent homodimers found in the mammalian counterpart. This may reduce the avidity of binding to individual ligand molecules expected to be present on Ag presenting cells. Peripheral + T cells are CD28-negative.1 Monoclonal antibodies against CD28 have a costimulatory effect on T cells stimulated by phorbol myristate acetate (PMA), concanavalin A or MAbs against the avian T cell receptor (TCR). Both V1- and V2-expressing cells respond equally well to stimulation with anti-CD28 in combination with PMA. These responses are resistant to cyclosporin A, but inhibited by herbimycin A, suggesting that CD28 employs a signalling pathway at least partly distinct from that triggered by TCR/CD3.

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response