OTUB1 antibody (AA 51-150) (Cy3)

Catalog No. ABIN1407927

Product Details anti-OTUB1 Antibody (Cy3)

Target: OTUB1 (OTU Domain, Ubiquitin Aldehyde Binding 1 (OTUB1))

Binding Specificity: AA 51-150

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This OTUB1 antibody is conjugated to Cy3

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Sheep,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human OTUB1

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for OTUB1

Target: OTUB1 (OTU Domain, Ubiquitin Aldehyde Binding 1 (OTUB1))

Alternative Name: OTUB1 (OTUB1 Products)

Background:

Synonyms: Deubiquitinating enzyme OTUB1, hOTU1, OTB1, OTU domain containing ubiquitin aldehyde binding protein 1, OTU domain-containing ubiquitin aldehyde-binding protein 1, OTU1, Otub1, OTUB1_HUMAN, Otubain 1, Otubain-1, Ubiquitin specic processing protease OTUB1, Ubiquitin thioesterase OTUB1, Ubiquitin-specic-processing protease OTUB1.

Background: OTUB1 is a 271 amino acid protein that contains one OTU (ovarian tumor) domain and belongs to the OTU family of predicted cysteine proteases. Expressed as two isoforms (one of which is present throughout the body and the other of which is present only in lymphoid tissues), OTUB1 functions as a hydrolase that can remove ubiquitin residues from target proteins, thereby preventing protein degradation and playing an important role in protein turnover. OTUB1 interacts with GRAIL and, via this interaction, plays a role in the regulation and the induction of T-cell anergy, a phenomenon that occurs when T-cells are rendered unresponsive to their cognate antigens. Due to its interaction with GRAIL, OTUB1 is an important regulator of immune responses in secondary lymphoid organs.