ATG5 Autophagy Related 5 Homolog (S. Cerevisiae) (ATG5) (N-Term), (AA 1-30) antibody

Details for Product No. ABIN1449605
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Antigen
Synonyms
ATG5, CG1643, DmAtg5, Dmel\\CG1643, atg5, apg5l, zgc:100934, ATATG5, AUTOPHAGY 5, MKP11.20, MKP11_20, APG5, APG5-LIKE, APG5L, ASP, hAPG5, 2010107M05Rik, 3110067M24Rik, AW319544, Apg5l, Atg5l, C88337,  ... show more
ATG5, CG1643, DmAtg5, Dmel\\CG1643, atg5, apg5l, zgc:100934, ATATG5, AUTOPHAGY 5, MKP11.20, MKP11_20, APG5, APG5-LIKE, APG5L, ASP, hAPG5, 2010107M05Rik, 3110067M24Rik, AW319544, Apg5l, Atg5l, C88337, Paddy show less
Epitope
N-Term, AA 1-30
(36), (16), (10), (9), (4), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(146), (69), (54), (23), (20), (13), (12), (7), (7), (6), (2), (1), (1), (1), (1)
Host
Rabbit
(105), (31), (12), (3), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(5), (5), (5), (4), (4), (4), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
(132), (75), (72), (22), (16), (10), (6), (4), (3), (3), (1)
Pubmed 19 references available
Quantity 0.4 mL
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Catalog No. ABIN1449605
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Immunogen KLH conjugated synthetic peptide between 1~30 amino acids from the N-term of Human APG5L Genename: ATG5
Specificity This antibody recognizes Human and Mouse ATG5 (N-term). Other species not tested.
Purification Saturated Ammonium Sulfate precipitation followed by dialysis against PBS
Alternative Name APG5L / ATG5
Background Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG5, required for autophagy, conjugates to ATG12 and associates with an isolation membrane to form a cup- shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. APG5 may also play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.
Alternate names: APG5-like, ASP, Apoptosis-specific protein, Autophagy protein 5
Gene ID 9474
NCBI Accession NP_004840
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS containing 0.09% (w/v) Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Background publications Grand, Milner, Mustoe et al.: "A novel protein expressed in mammalian cells undergoing apoptosis." in: Experimental cell research, Vol. 218, Issue 2, pp. 439-51, 1995 (PubMed).

Hammond, Brunet, Johnson et al.: "Homology between a human apoptosis specific protein and the product of APG5, a gene involved in autophagy in yeast." in: FEBS letters, Vol. 425, Issue 3, pp. 391-5, 1998 (PubMed).

Mizushima, Sugita, Yoshimori et al.: "A new protein conjugation system in human. The counterpart of the yeast Apg12p conjugation system essential for autophagy." in: The Journal of biological chemistry, Vol. 273, Issue 51, pp. 33889-92, 1999 (PubMed).

Mizushima, Yamamoto, Hatano et al.: "Dissection of autophagosome formation using Apg5-deficient mouse embryonic stem cells." in: The Journal of cell biology, Vol. 152, Issue 4, pp. 657-68, 2001 (PubMed).

Strausberg, Feingold, Grouse et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. ..." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).

Shintani, Klionsky: "Autophagy in health and disease: a double-edged sword." in: Science (New York, N.Y.), Vol. 306, Issue 5698, pp. 990-5, 2004 (PubMed).

Levine: "Eating oneself and uninvited guests: autophagy-related pathways in cellular defense." in: Cell, Vol. 120, Issue 2, pp. 159-62, 2005 (PubMed).

Lum, DeBerardinis, Thompson: "Autophagy in metazoans: cell survival in the land of plenty." in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 439-48, 2005 (PubMed).

Baehrecke: "Autophagy: dual roles in life and death?" in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 505-10, 2005 (PubMed).

Greenberg: "Degrade or die: a dual function for autophagy in the plant immune response." in: Developmental cell, Vol. 8, Issue 6, pp. 799-801, 2005 (PubMed).

Norberg, Gogvadze, Ott et al.: "An increase in intracellular Ca2+ is required for the activation of mitochondrial calpain to release AIF during cell death." in: Cell death and differentiation, Vol. 15, Issue 12, pp. 1857-64, 2008 (PubMed).

Xia, Zhang, Chen et al.: "Control of basal autophagy by calpain1 mediated cleavage of ATG5." in: Autophagy, Vol. 6, Issue 1, pp. 61-6, 2010 (PubMed).

Ferreri, Illerhaus, Zucca et al.: "Flows and flaws in primary central nervous system lymphoma." in: Nature reviews. Clinical oncology, Vol. 7, Issue 8, pp. doi:10.1038/nrclinonc.2010.9-c1; author reply doi:10:1038/nrclinonc.2010.9-c2, 2010 (PubMed).

Lépine, Allegood, Park et al.: "Sphingosine-1-phosphate phosphohydrolase-1 regulates ER stress-induced autophagy." in: Cell death and differentiation, Vol. 18, Issue 2, pp. 350-61, 2011 (PubMed).

Chiu, Ho, Wang: "Arsenic trioxide induces autophagy and apoptosis in human glioma cells in vitro and in vivo through downregulation of survivin." in: Journal of molecular medicine (Berlin, Germany), Vol. 89, Issue 9, pp. 927-41, 2011 (PubMed).

Lépine, Allegood, Edmonds et al.: "Autophagy induced by deficiency of sphingosine-1-phosphate phosphohydrolase 1 is switched to apoptosis by calpain-mediated autophagy-related gene 5 (Atg5) cleavage." in: The Journal of biological chemistry, Vol. 286, Issue 52, pp. 44380-90, 2011 (PubMed).

Keil, Höcker, Schuster et al.: "Phosphorylation of Atg5 by the Gadd45β-MEKK4-p38 pathway inhibits autophagy." in: Cell death and differentiation, Vol. 20, Issue 2, pp. 321-32, 2013 (PubMed).

Schober, Parlato, Huber et al.: "Cell loss and autophagy in the extra-adrenal chromaffin organ of Zuckerkandl are regulated by glucocorticoid signalling." in: Journal of neuroendocrinology, Vol. 25, Issue 1, pp. 34-47, 2013 (PubMed).

Huang, Chegini, Chua et al.: "Macroautophagy in sporadic and the genetic form of Parkinson's disease with the A53T α-synuclein mutation." in: Translational neurodegeneration, Vol. 1, Issue 1, pp. 2, 2012 (PubMed).

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