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Details for Product No. ABIN179725

BoNT B, Lightchain antibody

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Antigen
Reactivity
Clostridium botulinum (C. botulinum)
Host
Mouse (Murine)
Clonality (Clone) Monoclonal ()
Application
Flow Cytometry (FACS), Cell-ELISA (cELISA)
Pubmed 4 references available
Catalog no. ABIN179725
Quantity 100 µg
Price
450.12 $   Plus shipping costs $45.00
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Availability Will be delivered in 5 to 6 Business Days
Immunogen genetic immunisation with cDNA encoding BoNT/B
Clone GR-3G7
Isotype IgG1
Specificity Anti-BoNT/B
Purification Protein G
Background Botulinum neurotoxin type B (BoNT/B) is produced by Clostridium botulinum, a genetically diverse class of anaerobic, spore-forming, gram-positive bacilli. Seven different botulinum toxin groups have been identified serologically and are called botulinum toxin type A,B,C,D,E,F, and G. BoNT/B is a two-chain polypeptide with a 100-kDa heavy chain, which is responsible for neurospecific binding joined by a disulphide bond to a 50-kDa light chain, a zinc-endopeptidase which blocks neurotransmitter release. BoNT/B is one of the most poisonous naturally occurring substances. It inhibits acetylcholine release from neuromuscular junctions while it is used as an important therapeutic mainstay in the treatment of spasticity disorders and as a cosmetic treatment.
Application Notes Flow cytometry: 1.2 µg/10^6 cells
CELISA: 1:200 - 1:400
For each application a titration should be performed to determine the optimal concentration.
Restrictions For Research Use only
Concentration 2 mg/mL
Buffer PBS, pH 7.2
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C
Storage Comment short term: 2 °C - 8 °C, long term: -20 °C
Schiavo, Benfenati, Poulain et al.: "Tetanus and botulinum-B neurotoxins block neurotransmitter release by proteolytic cleavage of synaptobrevin." in: Nature, Vol. 359, Issue 6398, pp. 832-5, 1992 (PubMed).

DasGupta: "Structure and biological activity of botulinum neurotoxin." in: Journal de physiologie, Vol. 84, Issue 3, pp. 220-8, 1991 (PubMed).

Tonello, Morante, Rossetto et al.: "Tetanus and botulism neurotoxins: a novel group of zinc-endopeptidases." in: Advances in experimental medicine and biology, Vol. 389, pp. 251-60, 1997 (PubMed).

Papapetropoulos, Singer: "Botulinum toxin in movement disorders." in: Seminars in neurology, Vol. 27, Issue 2, pp. 183-94, 2007 (PubMed).

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