Botulinum Neurotoxin Type B (BoNT/B) antibody

Catalog No. ABIN1381702

This Chicken egg not_set antibody specifically detects Botulinum Neurotoxin Type B in ELISA and WB. It exhibits reactivity toward Clostridium botulinum.It has been mentioned in 1 publication

Quick Overview for Botulinum Neurotoxin Type B (BoNT/B) antibody (ABIN1381702)

Target: Botulinum Neurotoxin Type B (BoNT/B)

Reactivity: Clostridium botulinum

Host: Chicken egg

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Product Details

Characteristics: Chickens were immunized with highly purified Botulinum Neurotoxin Type B (Clostridium botulinum). After multiple immunizations in Freund's adjuvant, eggs were collected and the IgY purified. Anti-Botulinum Neurotoxin Type B was immunoaffinity purified using the immunogen immobilized on a solid support.

Purification: egg yolk-derived purified IgY, affinity purified

Application Details

Application Notes: This antibody is suitable for ELISA and Western Blotting procedures. For direct ELISA, a 1:66,000 dilution detects Botulinum HccB and a 1:42,000 dilutions detects Clostridium botulinum Type B (BTB). For Western blot, the recommended dilution is between 1:1000 and 1:2000. Optimal working dilutions should be determined for your particular assay conditions.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 2.0 mg/ml

Buffer: Phosphate Buffered Saline (PBS), pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References

Jenko, Zhang, Kostenko, Fan, Garcia-Rodriguez, Lou, Marks, Varnum: "Development of an ELISA microarray assay for the sensitive and simultaneous detection of ten biodefense toxins." in: The Analyst, Vol. 139, Issue 20, pp. 5093-102, (2015) (PubMed).

Target Details

Target: Botulinum Neurotoxin Type B (BoNT/B)

Alternative Name: Neurotoxin Type B

Target Type: Bacteria