Botulinum Neurotoxin Type B (BoNT/B) antibody

Catalog No. ABIN179725

Product Details

Target: Botulinum Neurotoxin Type B (BoNT/B)

Reactivity: Clostridium botulinum

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Cell-ELISA (cELISA), Flow Cytometry (FACS)

Specificity: Anti-BoNT/B

Purification: Protein G

Immunogen: genetic immunisation with cDNA encoding BoNT/B

Clone: GR-3G7

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: 1.2 µg/10^6 cells
CELISA: 1:200 - 1:400
For each application a titration should be performed to determine the optimal concentration.

Restrictions: For Research Use only

Handling

Concentration: 2 mg/mL

Buffer: PBS, pH 7.2

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C

Storage Comment: short term: 2 °C - 8 °C, long term: -20 °C

Target Details

Target: Botulinum Neurotoxin Type B (BoNT/B)

Alternative Name: BoNT B, Lightchain (BoNT/B Products)

Target Type: Bacteria

Background: Botulinum neurotoxin type B (BoNT/B) is produced by Clostridium botulinum, a genetically diverse class of anaerobic, spore-forming, gram-positive bacilli. Seven different botulinum toxin groups have been identified serologically and are called botulinum toxin type A,B,C,D,E,F, and G. BoNT/B is a two-chain polypeptide with a 100-kDa heavy chain, which is responsible for neurospecific binding joined by a disulphide bond to a 50-kDa light chain, a zinc-endopeptidase which blocks neurotransmitter release. BoNT/B is one of the most poisonous naturally occurring substances. It inhibits acetylcholine release from neuromuscular junctions while it is used as an important therapeutic mainstay in the treatment of spasticity disorders and as a cosmetic treatment.