Botulinum Neurotoxin Type B (BoNT/B) antibody

Antigen: Botulinum Neurotoxin Type B (BoNT/B)

Clonality: Monoclonal (GR-3G7)

Host: Mouse

Reactivity: Human

Application: Flow Cytometry (FACS), Cell-ELISA (cELISA)

Catalog no.: ABIN179725

Additional Information

Immunogen: genetic immunisation with cDNA encoding BoNT/B

Format: Purified

Isotype: IgG1

Clone: GR-3G7

Description: Botulinum neurotoxin type B (BoNT/B) is produced by Clostridium botulinum, a genetically diverse class of anaerobic, spore-forming, gram-positive bacilli. Seven different botulinum toxin groups have been identified serologically and are called botulinum toxin type A,B,C1,D,E,F, and G (1). BoNT/B is a two-chain polypeptide with a 100-kDa heavy chain, which is responsible for neurospecific binding joined by a disulphide bond to a 50-kDa light chain, a zinc-endopeptidase which blocks neurotransmitter release (2,3). BoNT/B is one of the most poisonous naturally occurring substances. It inhibits acetylcholine release from neuromuscular junctions while it is used as an important therapeutic mainstay in the treatment of spasticity disorders and as a cosmetic treatment (4).

Specificity: Anti-BoNT/B

Application Details

Application Notes: FACS: 1.2ug/10^6 cells, Cell-ELISA: 1:200 to 1:400

Concentration: 2 mg/ml

Purification: Protein G

Buffer: phosphate buffered saline, pH 7.2

Storage: Short term: 2°C - 8°C. long term: -20°C (avoid repeated freezing and thawing)

Restrictions: For Research Use only

Images

FACS analysis of BOSC23 cells using GR-3G7. BOSC23 cells were transiently trans-fected with an expression vector encoding eitherBoNT/B(red curve) or an irrelevant protein (controltransfectant). Binding of GR-3G7 was detected with a PE conjugated secondary antibody. A positive signal was obtained only with BoNT/B transfected cells.

BOSC23 cells were transiently transfected withexpression vectors containing the cDNA of the lightchain of botulinum toxin A-F. Expression of the constructs was tested with an anti-myc antibody (greencurves). An irrelevant monoclonal antibody served as an

SDS-PAGE analysis of purified GR-3G7 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ug ofpurified GR-3G7 antibody. Proteins were separated by SDS-PAGE and stained with RAPID Stain Reagent.

Publications

Schiavo, Benfenati, Poulain et al.: "Tetanus and botulinum-B neurotoxins block neurotransmitter release by proteolytic cleavage of synaptobrevin." in: Nature, Vol. 359, Issue 6398, pp. 832-5, 1992 (PubMed).

DasGupta: "Structure and biological activity of botulinum neurotoxin." in: Journal de physiologie, Vol. 84, Issue 3, pp. 220-8, 1991 (PubMed).

Tonello, Morante, Rossetto et al.: "Tetanus and botulism neurotoxins: a novel group of zinc-endopeptidases." in: Advances in experimental medicine and biology, Vol. 389, pp. 251-60, 1997 (PubMed).

Papapetropoulos, Singer: "Botulinum toxin in movement disorders." in: Seminars in neurology, Vol. 27, Issue 2, pp. 183-94, 2007 (PubMed).