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Botulinum Neurotoxin Type B (BoNT/B) antibody

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Antigen
Reactivity
Clostridium botulinum (C. botulinum)
(4)
Host
Mouse
(3), (1)
Clonality (Clone)
Monoclonal ()
Application
Cell-ELISA (cELISA), Flow Cytometry (FACS)
(2), (2), (1), (1), (1)
Pubmed 4 references available
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Quantity 100 μg
Shipping to United States ( )
Availability Will be delivered in 5 to 6 Business Days
Immunogen genetic immunisation with cDNA encoding BoNT/B
Clone GR-3G7
Isotype IgG1
Specificity Anti-BoNT/B
Purification Protein G
Alternative Name BoNT B, Lightchain (BoNT/B Antibody Abstract)
Background Botulinum neurotoxin type B (BoNT/B) is produced by Clostridium botulinum, a genetically diverse class of anaerobic, spore-forming, gram-positive bacilli. Seven different botulinum toxin groups have been identified serologically and are called botulinum toxin type A,B,C,D,E,F, and G. BoNT/B is a two-chain polypeptide with a 100-kDa heavy chain, which is responsible for neurospecific binding joined by a disulphide bond to a 50-kDa light chain, a zinc-endopeptidase which blocks neurotransmitter release. BoNT/B is one of the most poisonous naturally occurring substances. It inhibits acetylcholine release from neuromuscular junctions while it is used as an important therapeutic mainstay in the treatment of spasticity disorders and as a cosmetic treatment.
Pathways
Application Notes Flow cytometry: 1.2 µg/10^6 cells
CELISA: 1:200 - 1:400
For each application a titration should be performed to determine the optimal concentration.
Restrictions For Research Use only
Concentration 2 mg/mL
Buffer PBS, pH 7.2
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C
Storage Comment short term: 2 °C - 8 °C, long term: -20 °C
Supplier Images
 image for anti-Botulinum Neurotoxin Type B (BoNT/B) antibody (ABIN179725) FACS analysis of BOSC23 cells using GR-3G7. BOSC23 cells were transiently trans-fecte...
 image for anti-Botulinum Neurotoxin Type B (BoNT/B) antibody (ABIN179725) BOSC23 cells were transiently transfected withexpression vectors containing the cDNA ...
 image for anti-Botulinum Neurotoxin Type B (BoNT/B) antibody (ABIN179725) SDS-PAGE analysis of purified GR-3G7 monoclonal antibody. Lane 1: molecular weight ma...
Flow Cytometry (FACS) image for anti-Botulinum Neurotoxin Type B (BoNT/B) antibody (ABIN179725) FACS analysis of BOSC23 cells using GR-3G7. BOSC23 cells were transiently trans-fecte...
SDS-PAGE (SDS) image for anti-Botulinum Neurotoxin Type B (BoNT/B) antibody (ABIN179725) SDS-PAGE analysis of purified GR- 3G7 monoclonal antibody. Lane 1: molecular weight m...
Flow Cytometry (FACS) image for anti-Botulinum Neurotoxin Type B (BoNT/B) antibody (ABIN179725) BOSC23 cells were transiently transfected with expression vectors containing the cDNA...
Background publications Papapetropoulos, Singer: "Botulinum toxin in movement disorders." in: Seminars in neurology, Vol. 27, Issue 2, pp. 183-94, 2007 (PubMed).

Tonello, Morante, Rossetto et al.: "Tetanus and botulism neurotoxins: a novel group of zinc-endopeptidases." in: Advances in experimental medicine and biology, Vol. 389, pp. 251-60, 1997 (PubMed).

DasGupta: "Structure and biological activity of botulinum neurotoxin." in: Journal de physiologie, Vol. 84, Issue 3, pp. 220-8, 1991 (PubMed).

Schiavo, Benfenati, Poulain et al.: "Tetanus and botulinum-B neurotoxins block neurotransmitter release by proteolytic cleavage of synaptobrevin." in: Nature, Vol. 359, Issue 6398, pp. 832-5, 1992 (PubMed).

Catalog No. ABIN179725
$358.05
Plus shipping costs $45.00

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