IL-10 antibody

Catalog No. ABIN181890

Product Details anti-IL-10 Antibody

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Reactivity: Cow, Horse, Sheep, Goat

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL-10 antibody is un-conjugated

Application: Flow Cytometry (FACS), Enzyme Immunoassay (EIA), Functional Studies (Func)

Specificity: This antibody recognizes IL-10. Clone CC320 has been shown to neutralise the activity of bovine IL-10 as measured by the inhibition of the inhibitory activity of IL-10 on IFN gamma synthesis (Buza et al. 2004).

Purification: Affinity chromatography on Protein G

Endotoxin Level: < 0.01 EU/μg

Immunogen: Plasmid cDNA encoding bovine IL-10. Spleen cells from immunised Balb/c mice were fused with cells of the mouse sp2/0 myeloma cell line.

Clone: CC320

Isotype: IgG1

Application Details

Application Notes: Functional Assays. ELISA: 5 μg/mL - 10 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: Endotoxin Level: less than 0.01 EU/ug

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, None, None

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody at -20 °C. Ship at 2 - 8 °C.

Target Details for IL-10

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Alternative Name: Interleukin-10 / IL10 (IL10 Products)

Background: Interleukins (ILs) are a large group of cytokines that are produced mainly by leukocytes, although some are produced by certain phagocytes and auxiliary cells. ILs have a variety of functions, but most function to direct other immune cells to divide and differentiate. Each IL acts on a specific, limited group of cells through a receptor specific for that IL. Human IL10 is a non glycosylated polypeptide consisting of 178 amino acids. There is 73 % homology between the human and mouse IL10 proteins, however, the human IL10 acts on both human and mouse target cells, while the mouse IL10 has species specific activity. The cellular sources of IL10 are CD4+ T cells and T cell clones, thymocytes, B cells and B cell lymphomas, macrophages, mast cell lines and keratinocytes. IL10 will stimulate the growth of stem cells, mast cells and thymocytes. IL10 enhances cytotoxic T cell development, and costimulates B cell differentiation and immunoglobulin secretion. IL10 inhibits cytokine production by macrophages and suppresses macrophage class II MHC expression. The human IL10 gene is on human chromosome 1.Synonyms: CSIF, Cytokine synthesis inhibitory factor, IL-10, TGIF

Gene ID: 281246

NCBI Accession: NP_776513

UniProt: P43480

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints