C-Type Lectin Domain Family 7, Member A (CLEC7A) antibody

Details for Product No. ABIN181895
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Antigen
Synonyms CLEC7A, BGR, Clecsf12, beta-GR, CANDF4, CLECSF12, DECTIN1, Clec7b, RGD1565140
Reactivity
Mouse (Murine)
(53), (48), (20)
Host
Rat
(42), (22), (17)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(13), (9), (5), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Functional Studies (Func), Immunoprecipitation (IP)
(38), (28), (17), (10), (8), (7), (7), (7), (3), (2), (1), (1), (1)
Pubmed 7 references available
Quantity 0.5 mg
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Catalog No. ABIN181895
731.50 $
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Immunogen Dectin-1 transfected NIH3T3 cells and recombinant soluble Dectin-1. Spleen cells from immunised Fischer rats were fused with cells of the rat Y3 myeloma cell line.
Clone 2A11
Isotype IgG2b
Specificity This antibody clone 2A11 recognises beta-Glucan Receptor, also known as Dectin-1. This antibody inhibits the binding of zymosan to macrophages via the beta-glucan receptor. The low endotoxin version (SM2025LE) is recommended for functional studies.
Purification Affinity Chromatography on Protein G
Endotoxin Level Low Endotoxin
Alternative Name CLEC7A / Dectin-1
Background Dectin-1 is predominantly expressed by cells of the monocyte/macrophage and neutrophil lineages, but also at lower levels by dendritic cells and a subpopulation of T cells. As a major leucocyte receptor for beta-glucan this molecule may have a key role in the immunomodulatory effects of beta-glucans and in the host response to fungal pathogens. Recent work has shown that Dectin-1 may stimulate reactive oxygen production in macropahges via the protein tyrosine kinase known as Syk.Synonyms: BGR, Beta-glucan receptor, C-type lectin domain family 7 member A, C-type lectin superfamily member 12, CLECSF12, DC-associated C-type lectin 1, DECTIN1, Dendritic cell-associated C-type lectin 1
Gene ID 56644
NCBI Accession NP_064392
UniProt Q6QLQ4
Application Notes Functional Assays. Immunoprecipitation. Immunohistochemistry on frozen sections: The epitope recognised by 2A11 is sensitive to H202 treatment, therefore quenching with 0.3 % H202 should be performed after incubation with the primary antibody. Flow Cytometry: 1/10 - 1/50, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: Endotoxin Level: less than 0.01 EU/ug
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4, None, None
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
Supplier Images
anti-C-Type Lectin Domain Family 7, Member A (CLEC7A) antibody Staining of mouse peripheral blood monocytes with RAT ANTI MOUSE BETA GLUCAN RECEPTOR (ABIN181895).
Background publications Taylor, Brown, Reid et al.: "The beta-glucan receptor, dectin-1, is predominantly expressed on the surface of cells of the monocyte/macrophage and neutrophil lineages." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 169, Issue 7, pp. 3876-82, 2002 (PubMed).

Reid, Montoya, Taylor et al.: "Expression of the beta-glucan receptor, Dectin-1, on murine leukocytes in situ correlates with its function in pathogen recognition and reveals potential roles in leukocyte interactions." in: Journal of leukocyte biology, Vol. 76, Issue 1, pp. 86-94, 2004 (PubMed).

Underhill, Rossnagle, Lowell et al.: "Dectin-1 activates Syk tyrosine kinase in a dynamic subset of macrophages for reactive oxygen production." in: Blood, Vol. 106, Issue 7, pp. 2543-50, 2005 (PubMed).

Lefèvre, Galès, Olagnier et al.: "PPARγ ligands switched high fat diet-induced macrophage M2b polarization toward M2a thereby improving intestinal Candida elimination." in: PLoS ONE, Vol. 5, Issue 9, pp. e12828, 2010 (PubMed).

Gazi, Rosas, Singh et al.: "Fungal recognition enhances mannose receptor shedding through dectin-1 engagement." in: The Journal of biological chemistry, Vol. 286, Issue 10, pp. 7822-9, 2011 (PubMed).

McDonald, Cortini, Rosas et al.: "In vivo functional analysis and genetic modification of in vitro-derived mouse neutrophils." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 25, Issue 6, pp. 1972-82, 2011 (PubMed).

Fei, Bhatia, Oriss et al.: "TNF-alpha from inflammatory dendritic cells (DCs) regulates lung IL-17A/IL-5 levels and neutrophilia versus eosinophilia during persistent fungal infection." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 13, pp. 5360-5, 2011 (PubMed).

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