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C-Type Lectin Domain Family 7, Member A (CLEC7A) antibody

Details for Product No. ABIN181895, Supplier: Log in to see
Antigen
  • CLEC7A
  • BGR
  • Clecsf12
  • beta-GR
  • CANDF4
  • CLECSF12
  • DECTIN1
  • Clec7b
  • RGD1565140
Reactivity
Mouse (Murine)
108
90
19
4
1
Host
Rat
64
60
53
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
34
23
12
7
4
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
117
37
24
20
19
14
12
11
8
4
3
1
Supplier
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Immunogen Dectin-1 transfected NIH3T3 cells and recombinant soluble Dectin-1. Spleen cells from immunised Fischer rats were fused with cells of the rat Y3 myeloma cell line.
Clone 2A11
Isotype IgG2b
Specificity This antibody clone 2A11 recognises beta-Glucan Receptor, also known as Dectin-1. This antibody inhibits the binding of zymosan to macrophages via the beta-glucan receptor. The low endotoxin version (SM2025LE) is recommended for functional studies.
Purification Affinity Chromatography on Protein G
Endotoxin Level Low Endotoxin
Alternative Name CLEC7A / Dectin-1 (CLEC7A Antibody Abstract)
Background Dectin-1 is predominantly expressed by cells of the monocyte/macrophage and neutrophil lineages, but also at lower levels by dendritic cells and a subpopulation of T cells. As a major leucocyte receptor for beta-glucan this molecule may have a key role in the immunomodulatory effects of beta-glucans and in the host response to fungal pathogens. Recent work has shown that Dectin-1 may stimulate reactive oxygen production in macropahges via the protein tyrosine kinase known as Syk.Synonyms: BGR, Beta-glucan receptor, C-type lectin domain family 7 member A, C-type lectin superfamily member 12, CLECSF12, DC-associated C-type lectin 1, DECTIN1, Dendritic cell-associated C-type lectin 1
Gene ID 56644
NCBI Accession NP_064392
UniProt Q6QLQ4
Pathways
Application Notes Functional Assays. Immunoprecipitation. Immunohistochemistry on frozen sections: The epitope recognised by 2A11 is sensitive to H202 treatment, therefore quenching with 0.3 % H202 should be performed after incubation with the primary antibody. Flow Cytometry: 1/10 - 1/50, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: Endotoxin Level: less than 0.01 EU/ug
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4, None, None
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
Supplier Images
Flow Cytometry (FACS) image for anti-C-Type Lectin Domain Family 7, Member A (CLEC7A) antibody (ABIN181895) Staining of mouse peripheral blood monocytes with RAT ANTI MOUSE BETA GLUCAN RECEPTOR...
Background publications Fei, Bhatia, Oriss et al.: "TNF-alpha from inflammatory dendritic cells (DCs) regulates lung IL-17A/IL-5 levels and neutrophilia versus eosinophilia during persistent fungal infection." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 13, pp. 5360-5, 2011 (PubMed).

McDonald, Cortini, Rosas et al.: "In vivo functional analysis and genetic modification of in vitro-derived mouse neutrophils." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 25, Issue 6, pp. 1972-82, 2011 (PubMed).

Gazi, Rosas, Singh et al.: "Fungal recognition enhances mannose receptor shedding through dectin-1 engagement." in: The Journal of biological chemistry, Vol. 286, Issue 10, pp. 7822-9, 2011 (PubMed).

Lefèvre, Galès, Olagnier et al.: "PPARγ ligands switched high fat diet-induced macrophage M2b polarization toward M2a thereby improving intestinal Candida elimination." in: PLoS ONE, Vol. 5, Issue 9, pp. e12828, 2010 (PubMed).

Underhill, Rossnagle, Lowell et al.: "Dectin-1 activates Syk tyrosine kinase in a dynamic subset of macrophages for reactive oxygen production." in: Blood, Vol. 106, Issue 7, pp. 2543-50, 2005 (PubMed).

Reid, Montoya, Taylor et al.: "Expression of the beta-glucan receptor, Dectin-1, on murine leukocytes in situ correlates with its function in pathogen recognition and reveals potential roles in leukocyte interactions." in: Journal of leukocyte biology, Vol. 76, Issue 1, pp. 86-94, 2004 (PubMed).

Taylor, Brown, Reid et al.: "The beta-glucan receptor, dectin-1, is predominantly expressed on the surface of cells of the monocyte/macrophage and neutrophil lineages." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 169, Issue 7, pp. 3876-82, 2002 (PubMed).